Giuseppe
Emeritus
Visual Detection of Pandemic Influenza A H1N1 Virus 2009 by Reverse Transcription Loop-Mediated Isothermal Amplification with Hydroxynaphthol Blue Dye. (J Virol Methods, abstract, edited)
[Source: US National Library of Medicine, (LINK). Edited.]
J Virol Methods. 2010 Apr 6. [Epub ahead of print]
Visual Detection of Pandemic Influenza A H1N1 Virus 2009 by Reverse Transcription Loop-Mediated Isothermal Amplification with Hydroxynaphthol Blue Dye.
Ma XJ, Shu YL, Nie K, Wang MQ, Gao RB, Wang M, Wen LY, Han F, Zhou SM, Zhao X, Cheng YH, Li DX, Dong XP. - National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Rd 100, Xuan Wu District, Beijing, 100052, China.
A sensitive reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for rapid visual detection of pandemic influenza A H1N1 virus infection. The reaction was performed in one step in a single tube at 65(o)C for 60min with the addition of hydroxynaphthol blue (HNB) dye prior to amplification. The detection limit of the RT-LAMP assay was approximately 60 copies, and no cross-detection was observed. The assay was evaluated further with 50 clinical specimens diagnosed clinically with seasonal influenza or pandemic influenza A H1N1 virus infection. RT-LAMP with HNB dye was demonstrated to be a sensitive and easy assay for rapid detection of pandemic influenza A H1N1 virus.
Copyright ? 2010. Published by Elsevier B.V.
PMID: 20381535 [PubMed - as supplied by publisher]
-
------
[Source: US National Library of Medicine, (LINK). Edited.]
J Virol Methods. 2010 Apr 6. [Epub ahead of print]
Visual Detection of Pandemic Influenza A H1N1 Virus 2009 by Reverse Transcription Loop-Mediated Isothermal Amplification with Hydroxynaphthol Blue Dye.
Ma XJ, Shu YL, Nie K, Wang MQ, Gao RB, Wang M, Wen LY, Han F, Zhou SM, Zhao X, Cheng YH, Li DX, Dong XP. - National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Rd 100, Xuan Wu District, Beijing, 100052, China.
A sensitive reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for rapid visual detection of pandemic influenza A H1N1 virus infection. The reaction was performed in one step in a single tube at 65(o)C for 60min with the addition of hydroxynaphthol blue (HNB) dye prior to amplification. The detection limit of the RT-LAMP assay was approximately 60 copies, and no cross-detection was observed. The assay was evaluated further with 50 clinical specimens diagnosed clinically with seasonal influenza or pandemic influenza A H1N1 virus infection. RT-LAMP with HNB dye was demonstrated to be a sensitive and easy assay for rapid detection of pandemic influenza A H1N1 virus.
Copyright ? 2010. Published by Elsevier B.V.
PMID: 20381535 [PubMed - as supplied by publisher]
-
------