tetano
Editor, Senior Moderator
J Virol Methods
. 2022 Jan 13;114465.
doi: 10.1016/j.jviromet.2022.114465. Online ahead of print.
Rapid thermal inactivation of aerosolized SARS-CoV-2
Murat Canpolat[SUP] 1 [/SUP], Serhat Bozkurt[SUP] 2 [/SUP], Çağrı Şakalar[SUP] 3 [/SUP], Ahmet Yılmaz Çoban[SUP] 4 [/SUP], Deniz Karaçaylı[SUP] 5 [/SUP], Emre Toker[SUP] 6 [/SUP]
Affiliations
Abstract
Airborne transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is one of the leading mechanisms of spread, especially in confined environments. The study aims to assess the thermal inactivation of SARS-CoV-2 at high temperatures in the time scale of seconds. An electric heater with a coiled resistance wire is located perpendicularly to the airflow direction inside an air tunnel. The airflow rate through the tunnel was 0.6 m[SUP]3[/SUP]/h (10 L/ min). SARS-CoV-2 were suspended in Dulbecco's modified Eagle's medium (DMEM) with 10 % fetal bovine serum (FBS), aerosolized by a nebulizer at a rate of 0.2 L/min and introduced to the airflow inside the heater with the use of a compressor and an aspirator. In the control experiment, with the heater off, SARS-CoV-2 passed through the system. In the virus inactivation test experiments, the heater's outlet air temperature was set to 150 ± 5 °C and 220 ± 5 °C, and the air traveling through the tunnel was exposed to heat for 1.44 s. An inline gelatine filter harvested SARS-CoV-2 that passed through the system. The viral titer obtained from the gelatine filter in the control experiment was about 5.5 log[SUB]10[/SUB] TCID[SUB]50[/SUB]. The virus's loss in viability in test experiments at 150 °C and 220 °C were 99.900 % and 99.999 %, respectively. The results indicate that high-temperature thermal inactivation substantially reduces the concentration of SARS-CoV-2 in the air within seconds.
Keywords: Aerosolized coronavirus; COVID-19; High temperature; Rapid thermal inactivation; SARS-CoV-2.
. 2022 Jan 13;114465.
doi: 10.1016/j.jviromet.2022.114465. Online ahead of print.
Rapid thermal inactivation of aerosolized SARS-CoV-2
Murat Canpolat[SUP] 1 [/SUP], Serhat Bozkurt[SUP] 2 [/SUP], Çağrı Şakalar[SUP] 3 [/SUP], Ahmet Yılmaz Çoban[SUP] 4 [/SUP], Deniz Karaçaylı[SUP] 5 [/SUP], Emre Toker[SUP] 6 [/SUP]
Affiliations
- PMID: 35033579
- DOI: 10.1016/j.jviromet.2022.114465
Abstract
Airborne transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is one of the leading mechanisms of spread, especially in confined environments. The study aims to assess the thermal inactivation of SARS-CoV-2 at high temperatures in the time scale of seconds. An electric heater with a coiled resistance wire is located perpendicularly to the airflow direction inside an air tunnel. The airflow rate through the tunnel was 0.6 m[SUP]3[/SUP]/h (10 L/ min). SARS-CoV-2 were suspended in Dulbecco's modified Eagle's medium (DMEM) with 10 % fetal bovine serum (FBS), aerosolized by a nebulizer at a rate of 0.2 L/min and introduced to the airflow inside the heater with the use of a compressor and an aspirator. In the control experiment, with the heater off, SARS-CoV-2 passed through the system. In the virus inactivation test experiments, the heater's outlet air temperature was set to 150 ± 5 °C and 220 ± 5 °C, and the air traveling through the tunnel was exposed to heat for 1.44 s. An inline gelatine filter harvested SARS-CoV-2 that passed through the system. The viral titer obtained from the gelatine filter in the control experiment was about 5.5 log[SUB]10[/SUB] TCID[SUB]50[/SUB]. The virus's loss in viability in test experiments at 150 °C and 220 °C were 99.900 % and 99.999 %, respectively. The results indicate that high-temperature thermal inactivation substantially reduces the concentration of SARS-CoV-2 in the air within seconds.
Keywords: Aerosolized coronavirus; COVID-19; High temperature; Rapid thermal inactivation; SARS-CoV-2.