tetano
Editor, Senior Moderator
J Virol Methods
. 2021 Jan 13;114064.
doi: 10.1016/j.jviromet.2021.114064. Online ahead of print.
NUCLEOPROTEIN-BASED ELISA FOR DETECTION OF SARS-CoV-2 IgG ANTIBODIES: COULD AN OLD ASSAY BE SUITABLE FOR SERODIAGNOSIS OF THE NEW CORONAVIRUS?
Tania Regina Tozetto-Mendoza[SUP] 1 [/SUP], Kelly Aparecida Kanunfre[SUP] 2 [/SUP], Lucy Santos Vilas-Boas[SUP] 3 [/SUP], Evelyn Patricia Sanchez Espinoza[SUP] 4 [/SUP], Heuder Gustavo Oliveira Pai?o[SUP] 5 [/SUP], Mussya Cisotto Rocha[SUP] 2 [/SUP], Anderson Vicente de Paula[SUP] 3 [/SUP], Maura Salaroli de Oliveira[SUP] 6 [/SUP], Daniella Bosco Zampelli[SUP] 7 [/SUP], Jos? Mauro Vieira Jr[SUP] 7 [/SUP], Lewis Buss[SUP] 8 [/SUP], Silvia Figueiredo Costa[SUP] 4 [/SUP], Ester Cerdeira Sabino[SUP] 9 [/SUP], Steven S Witkin[SUP] 10 [/SUP], Thelma Suely Okay[SUP] 11 [/SUP], Maria Cassia Mendes-Correa[SUP] 5 [/SUP]
Affiliations
Abstract
Objectives: We evaluated the performance of a nucleoprotein-based enzyme-linked immunosorbent assay (ELISA) for detection of IgG antibodies to SARS-CoV-2.
Methods: The ELISA was based on serum IgG reactivity to a 46-kDa protein derived from the recombinant SARS-CoV2 nucleoprotein. Assay sensitivity was assessed using serum samples from 134 COVID-19 confirmed cases obtained > 15 days after symptom onset. Specificity was determined by testing sera from 94 healthy controls. Cross-reactivity was evaluated with sera from 96 individuals with previous dengue or zika virus-confirmed infections, with 44 sera from individuals with confirmed infections to other respiratory viruses or with bacterial and fungal infections that cause pneumonia and with 40 sera negative for SARS-CoV-2 nucleoprotein by commercial ELISA kits.
Results: The majority of subjects were male and> 60 years old. Assay sensitivity was 90.3% (95% confidence interval 84.1% - 94.2%) and specificity was 97.9% (92.6% - 99.4%). There was no cross-reactivity with sera from individuals diagnosed with dengue, zika virus, influenza virus, rhinovirus, adenovirus, respiratory syncytial virus, seasonal coronavirus, Mycobacterium tuberculosis, Staphylococcus (S. aureus and coagulase-negative), Streptococcus pneumoniae, Klebsiella pneumoniae and the fungus Aspergillus fumigatus. The level of concordance of our test with results from commercial ELISA kits was 100%.
Conclusion: The nucleoprotein-based ELISA was specific for detection of IgG anti-nucleoprotein antibodies to SARS-CoV-2.. It utilizes a frequently employed low expense assay protocol and is easier to perform than other currently available commercial SARS-CoV2 antibody detection tests.
Keywords: COVID-19; ELISA; IgG antibody; Nucleoprotein antigen; SARS-CoV-2.
. 2021 Jan 13;114064.
doi: 10.1016/j.jviromet.2021.114064. Online ahead of print.
NUCLEOPROTEIN-BASED ELISA FOR DETECTION OF SARS-CoV-2 IgG ANTIBODIES: COULD AN OLD ASSAY BE SUITABLE FOR SERODIAGNOSIS OF THE NEW CORONAVIRUS?
Tania Regina Tozetto-Mendoza[SUP] 1 [/SUP], Kelly Aparecida Kanunfre[SUP] 2 [/SUP], Lucy Santos Vilas-Boas[SUP] 3 [/SUP], Evelyn Patricia Sanchez Espinoza[SUP] 4 [/SUP], Heuder Gustavo Oliveira Pai?o[SUP] 5 [/SUP], Mussya Cisotto Rocha[SUP] 2 [/SUP], Anderson Vicente de Paula[SUP] 3 [/SUP], Maura Salaroli de Oliveira[SUP] 6 [/SUP], Daniella Bosco Zampelli[SUP] 7 [/SUP], Jos? Mauro Vieira Jr[SUP] 7 [/SUP], Lewis Buss[SUP] 8 [/SUP], Silvia Figueiredo Costa[SUP] 4 [/SUP], Ester Cerdeira Sabino[SUP] 9 [/SUP], Steven S Witkin[SUP] 10 [/SUP], Thelma Suely Okay[SUP] 11 [/SUP], Maria Cassia Mendes-Correa[SUP] 5 [/SUP]
Affiliations
- PMID: 33453299
- DOI: 10.1016/j.jviromet.2021.114064
Abstract
Objectives: We evaluated the performance of a nucleoprotein-based enzyme-linked immunosorbent assay (ELISA) for detection of IgG antibodies to SARS-CoV-2.
Methods: The ELISA was based on serum IgG reactivity to a 46-kDa protein derived from the recombinant SARS-CoV2 nucleoprotein. Assay sensitivity was assessed using serum samples from 134 COVID-19 confirmed cases obtained > 15 days after symptom onset. Specificity was determined by testing sera from 94 healthy controls. Cross-reactivity was evaluated with sera from 96 individuals with previous dengue or zika virus-confirmed infections, with 44 sera from individuals with confirmed infections to other respiratory viruses or with bacterial and fungal infections that cause pneumonia and with 40 sera negative for SARS-CoV-2 nucleoprotein by commercial ELISA kits.
Results: The majority of subjects were male and> 60 years old. Assay sensitivity was 90.3% (95% confidence interval 84.1% - 94.2%) and specificity was 97.9% (92.6% - 99.4%). There was no cross-reactivity with sera from individuals diagnosed with dengue, zika virus, influenza virus, rhinovirus, adenovirus, respiratory syncytial virus, seasonal coronavirus, Mycobacterium tuberculosis, Staphylococcus (S. aureus and coagulase-negative), Streptococcus pneumoniae, Klebsiella pneumoniae and the fungus Aspergillus fumigatus. The level of concordance of our test with results from commercial ELISA kits was 100%.
Conclusion: The nucleoprotein-based ELISA was specific for detection of IgG anti-nucleoprotein antibodies to SARS-CoV-2.. It utilizes a frequently employed low expense assay protocol and is easier to perform than other currently available commercial SARS-CoV2 antibody detection tests.
Keywords: COVID-19; ELISA; IgG antibody; Nucleoprotein antigen; SARS-CoV-2.