Giuseppe
Emeritus
Field detection of avian influenza virus in wild birds: evaluation of a portable RRT-PCR system and freeze dried reagents. (J Virol Methods., abstract, edited)
[Source: US National Library of Medicine, LINK. EDITED.]
J Virol Methods. 2010 Mar 3. [Epub ahead of print]
Field detection of avian influenza virus in wild birds: evaluation of a portable RRT-PCR system and freeze dried reagents.
Takekawa JY, Iverson SA, Schultz AK, Hill NJ, Cardona CJ, Boyce WM, Dudley JP. - U.S. Geological Survey, Western Ecological Research Center, San Francisco Bay Estuary Field Station, 505 Azuar Drive, Vallejo, CA 94592, USA.
Wild birds have been implicated in the spread of highly pathogenic avian influenza (HPAIV) of the H5N1 subtype, prompting surveillance along migratory flyways. Sampling of wild birds is often conducted in remote regions, but results are often delayed because of limited local analytical capabilities, difficulties with sample transportation and permitting, or problems keeping samples cold in the field. In response to these challenges, the performance of a portable realtime, reverse transcriptase-polymerase chain reaction (rRT-PCR) unit (RAPID((R)), Idaho Technologies, Salt Lake City, UT) that employed lyophilized reagents (Influenza A Target 1 Taqman; ASAY-ASY-0109, Idaho Technologies) was compared to virus isolation combined with real-time RT-PCR conducted in a laboratory. This study included both field- and experimental-based sampling. Field samples were collected from migratory shorebirds captured in northern California, while experimental samples were prepared by spiking fecal material with an H6N2 AIV isolate. Results indicated that the portable rRT-PCR unit had equivalent specificity to virus isolation with no false positives, but sensitivity was compromised at low viral titers. Use of portable rRT-PCR with lyophilized reagents may expedite surveillance results, paving the way to a better understanding of wild bird involvement in HPAIV H5N1 transmission.
Copyright ?2010. Published by Elsevier B.V.
PMID: 20206650 [PubMed - as supplied by publisher]
J Virol Methods. 2010 Mar 3. [Epub ahead of print]
Field detection of avian influenza virus in wild birds: evaluation of a portable RRT-PCR system and freeze dried reagents.
Takekawa JY, Iverson SA, Schultz AK, Hill NJ, Cardona CJ, Boyce WM, Dudley JP. - U.S. Geological Survey, Western Ecological Research Center, San Francisco Bay Estuary Field Station, 505 Azuar Drive, Vallejo, CA 94592, USA.
Wild birds have been implicated in the spread of highly pathogenic avian influenza (HPAIV) of the H5N1 subtype, prompting surveillance along migratory flyways. Sampling of wild birds is often conducted in remote regions, but results are often delayed because of limited local analytical capabilities, difficulties with sample transportation and permitting, or problems keeping samples cold in the field. In response to these challenges, the performance of a portable realtime, reverse transcriptase-polymerase chain reaction (rRT-PCR) unit (RAPID((R)), Idaho Technologies, Salt Lake City, UT) that employed lyophilized reagents (Influenza A Target 1 Taqman; ASAY-ASY-0109, Idaho Technologies) was compared to virus isolation combined with real-time RT-PCR conducted in a laboratory. This study included both field- and experimental-based sampling. Field samples were collected from migratory shorebirds captured in northern California, while experimental samples were prepared by spiking fecal material with an H6N2 AIV isolate. Results indicated that the portable rRT-PCR unit had equivalent specificity to virus isolation with no false positives, but sensitivity was compromised at low viral titers. Use of portable rRT-PCR with lyophilized reagents may expedite surveillance results, paving the way to a better understanding of wild bird involvement in HPAIV H5N1 transmission.
Copyright ?2010. Published by Elsevier B.V.
PMID: 20206650 [PubMed - as supplied by publisher]