Giuseppe
Emeritus
J Virol Methods. 2009 Jun 10. [Epub ahead of print]
Evaluation of an Epitope-Blocking Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies to Influenza A Virus in Domestic and Wild Avian and Mammalian Species.
Sullivan HJ, Blitvich BJ, Vandalen K, Bentler KT, Franklin AB, Root JJ. - United States Department of Agriculture, Wildlife Services, National Wildlife Research Center, Fort Collins, Colorado, 80521, USA.
An epitope-blocking enzyme-linked immunosorbent assay (bELISA) was developed for the detection of antibodies to influenza A virus in taxonomically diverse domestic and wild vertebrate species. In contrast to the bELISAs published previously that require reagent production, manipulation by the end-user, or have not been evaluated for use with both mammalian and avian species, this assay is performed using commercially available recombinant nucleoprotein antigen and corresponding nucleoprotein-specific monoclonal antibody and has been shown to work with multiple avian and mammalian species. The efficacy of the bELISA as a serum screening assay was compared to the agar gel immunodiffusion (AGID) assay using 251 serum samples obtained from experimentally-infected mallards(Anas platyrhynchos) and raccoons (Procyon lotor). The concordance between the AGID assay and bELISA was 94.1% (95% CI = 89.9, 98.3) for raccoons, and 71.2% (95% CI = 63.5, 78.9) for mallards and 82.8% (95% CI = 78.2, 87.3) overall. The bELISA was more sensitive than the AGID assay as demonstrated by the detection of antibodies to influenza A virus at earlier time points in experimental infection studies and at higher serial dilutions. The efficacy of the bELISA to monitor natural influenza A virus exposure was also compared to the AGID assay using an additional 745 serum samples from six avian species and six mammalian species. This bELISA provides a rapid, reliable, and inexpensive technique for large-scale surveillance of influenza A virus exposure in taxonomically diverse vertebrate species.
PMID: 19523985 [PubMed - as supplied by publisher]
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Evaluation of an Epitope-Blocking Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies to Influenza A Virus in Domestic and Wild Avian and Mammalian Species.
Sullivan HJ, Blitvich BJ, Vandalen K, Bentler KT, Franklin AB, Root JJ. - United States Department of Agriculture, Wildlife Services, National Wildlife Research Center, Fort Collins, Colorado, 80521, USA.
An epitope-blocking enzyme-linked immunosorbent assay (bELISA) was developed for the detection of antibodies to influenza A virus in taxonomically diverse domestic and wild vertebrate species. In contrast to the bELISAs published previously that require reagent production, manipulation by the end-user, or have not been evaluated for use with both mammalian and avian species, this assay is performed using commercially available recombinant nucleoprotein antigen and corresponding nucleoprotein-specific monoclonal antibody and has been shown to work with multiple avian and mammalian species. The efficacy of the bELISA as a serum screening assay was compared to the agar gel immunodiffusion (AGID) assay using 251 serum samples obtained from experimentally-infected mallards(Anas platyrhynchos) and raccoons (Procyon lotor). The concordance between the AGID assay and bELISA was 94.1% (95% CI = 89.9, 98.3) for raccoons, and 71.2% (95% CI = 63.5, 78.9) for mallards and 82.8% (95% CI = 78.2, 87.3) overall. The bELISA was more sensitive than the AGID assay as demonstrated by the detection of antibodies to influenza A virus at earlier time points in experimental infection studies and at higher serial dilutions. The efficacy of the bELISA to monitor natural influenza A virus exposure was also compared to the AGID assay using an additional 745 serum samples from six avian species and six mammalian species. This bELISA provides a rapid, reliable, and inexpensive technique for large-scale surveillance of influenza A virus exposure in taxonomically diverse vertebrate species.
PMID: 19523985 [PubMed - as supplied by publisher]
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