Giuseppe
Emeritus
Intra-host evolutionary dynamics of canine influenza virus in naive and partially immune dogs. (J Virol., abstract, edited)
[Source: US National Library of Medicine, LINK. EDITED.]
J Virol. 2010 Mar 10. [Epub ahead of print]
Intra-host evolutionary dynamics of canine influenza virus in naive and partially immune dogs.
Hoelzer K, Murcia PR, Baillie GJ, Wood JL, Metzger SM, Osterrieder N, Dubovi EJ, Holmes EC, Parrish CR. - Baker Institute of Animal Health, Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA; Cambridge Infectious Diseases Consortium, Department of Veterinary Medicine, Cambridge, CB3 0ES, United Kingdom; Wellcome Trust Sanger Institute. Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, United Kingdom; Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA; Institute f?r Virologie, Freie Universit?t Berlin, Philippstrasse 13, 10115 Berlin, Germany; Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA; Center for Infectious Disease Dynamics, Department of Biology, Pennsylvania State University, State College, PA 16802, USA and Fogarty International Center, National Institutes of Health, Bethesda, MD 20892, USA; Fogarty International Center, National Institutes of Health, Bethesda, MD 20892, USA.
The patterns and dynamics of evolution in acutely infecting viruses within individual hosts are largely unknown. To this end we investigated the intra-host variation of canine influenza virus (CIV) during the course of experimental infections in na?ve and partially immune dogs, and in naturally infected animals. Tracing sequence diversity in the gene encoding domain 1 of the hemagglutinin (HA1) protein over the time course of infection provided information on the patterns and processes of intra-host viral evolution, and revealed some of the effects of partial host immunity. Viral populations sampled on any given day were generally characterized by mean pairwise genetic diversities between 0.1 - 0.2%, and by mutational spectra that changed considerably between days. Some observed mutations may have affected antigenicity or host range, including reversions of CIV host-associated mutations. Patterns of sequence diversity differed between na?ve and vaccinated dogs, with some presumably antigenic mutations transiently reaching high frequency in the latter. CIV populations are therefore characterized by the rapid generation and clearance of genetic diversity. Potentially advantageous mutations arise readily during the course of single infections, and may give rise to antigenic escape or host range variants.
PMID: 20219907 [PubMed - as supplied by publisher]
J Virol. 2010 Mar 10. [Epub ahead of print]
Intra-host evolutionary dynamics of canine influenza virus in naive and partially immune dogs.
Hoelzer K, Murcia PR, Baillie GJ, Wood JL, Metzger SM, Osterrieder N, Dubovi EJ, Holmes EC, Parrish CR. - Baker Institute of Animal Health, Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA; Cambridge Infectious Diseases Consortium, Department of Veterinary Medicine, Cambridge, CB3 0ES, United Kingdom; Wellcome Trust Sanger Institute. Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, United Kingdom; Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA; Institute f?r Virologie, Freie Universit?t Berlin, Philippstrasse 13, 10115 Berlin, Germany; Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA; Center for Infectious Disease Dynamics, Department of Biology, Pennsylvania State University, State College, PA 16802, USA and Fogarty International Center, National Institutes of Health, Bethesda, MD 20892, USA; Fogarty International Center, National Institutes of Health, Bethesda, MD 20892, USA.
The patterns and dynamics of evolution in acutely infecting viruses within individual hosts are largely unknown. To this end we investigated the intra-host variation of canine influenza virus (CIV) during the course of experimental infections in na?ve and partially immune dogs, and in naturally infected animals. Tracing sequence diversity in the gene encoding domain 1 of the hemagglutinin (HA1) protein over the time course of infection provided information on the patterns and processes of intra-host viral evolution, and revealed some of the effects of partial host immunity. Viral populations sampled on any given day were generally characterized by mean pairwise genetic diversities between 0.1 - 0.2%, and by mutational spectra that changed considerably between days. Some observed mutations may have affected antigenicity or host range, including reversions of CIV host-associated mutations. Patterns of sequence diversity differed between na?ve and vaccinated dogs, with some presumably antigenic mutations transiently reaching high frequency in the latter. CIV populations are therefore characterized by the rapid generation and clearance of genetic diversity. Potentially advantageous mutations arise readily during the course of single infections, and may give rise to antigenic escape or host range variants.
PMID: 20219907 [PubMed - as supplied by publisher]