Giuseppe
Emeritus
Human RNA Polymerase I-Driven Reverse Genetics for Influenza A Virus in Canine Cells. (J Virol., abstract, edited)
J Virol. 2010 Jan 13. [Epub ahead of print]
Human RNA Polymerase I-Driven Reverse Genetics for Influenza A Virus in Canine Cells.
Suphaphiphat P, Keiner B, Trusheim H, Crotta S, Tuccino AB, Zhang P, Dormitzer PR, Mason PW, Franti M. - Novartis Vaccines and Diagnostics, 350 Massachusetts Ave., Cambridge, MA 02139, USA.
We have established a human RNA polymerase I (pol I)-driven influenza virus reverse genetics (RG) system in the Madin-Darby canine kidney 33016-PF cell line, which is approved for influenza vaccine manufacture. RNA pol I polymerases are generally active only in cells of species closely related to the species of origin of the polymerases. Nevertheless, we show that a non-endogenous RNA pol I promoter drives efficient rescue of influenza A viruses in a canine cell line. Application of this system allows efficient generation of virus strains and presents an alternative approach for influenza vaccine production.
PMID: 20071567 [PubMed - as supplied by publisher]
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J Virol. 2010 Jan 13. [Epub ahead of print]
Human RNA Polymerase I-Driven Reverse Genetics for Influenza A Virus in Canine Cells.
Suphaphiphat P, Keiner B, Trusheim H, Crotta S, Tuccino AB, Zhang P, Dormitzer PR, Mason PW, Franti M. - Novartis Vaccines and Diagnostics, 350 Massachusetts Ave., Cambridge, MA 02139, USA.
We have established a human RNA polymerase I (pol I)-driven influenza virus reverse genetics (RG) system in the Madin-Darby canine kidney 33016-PF cell line, which is approved for influenza vaccine manufacture. RNA pol I polymerases are generally active only in cells of species closely related to the species of origin of the polymerases. Nevertheless, we show that a non-endogenous RNA pol I promoter drives efficient rescue of influenza A viruses in a canine cell line. Application of this system allows efficient generation of virus strains and presents an alternative approach for influenza vaccine production.
PMID: 20071567 [PubMed - as supplied by publisher]
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