Giuseppe
Emeritus
J Virol. 2009 Nov 25. [Epub ahead of print]
Hemagglutinin-pseudotyped GFP-expressing influenza viruses for the detection of influenza virus neutralizing antibodies.
Mart?nez-Sobrido L, Cadagan R, Steel J, Basler CF, Palese P, Moran TM, Garc?a-Sastre A. - Departments of Microbiology, and Medicine, and Global Health and Emerging Pathogens Institute, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, NY 10029, USA.
Influenza virus is a highly contagious virus that causes yearly epidemics and occasional pandemics of great consequence. Influenza virus-neutralizing antibodies (NAbs) are promising prophylactic and therapeutic reagents. Detection of NAbs in serum samples is critical to evaluate the prevalence and spread of new virus strains. Here we describe the development of a simple, sensitive, specific and safe screening assay for the rapid detection of NAbs against highly pathogenic influenza viruses under BSL-2 conditions. This assay is based on the use of influenza viruses in which the hemagglutinin (HA) gene is replaced by one expressing GFP. These GFP-expressing influenza viruses replicate to high titers in HA-expressing cell lines, but in non-HA expressing cells their replication is restricted to a single cycle.
PMID: 19939917 [PubMed - as supplied by publisher]
-
------
Hemagglutinin-pseudotyped GFP-expressing influenza viruses for the detection of influenza virus neutralizing antibodies.
Mart?nez-Sobrido L, Cadagan R, Steel J, Basler CF, Palese P, Moran TM, Garc?a-Sastre A. - Departments of Microbiology, and Medicine, and Global Health and Emerging Pathogens Institute, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, NY 10029, USA.
Influenza virus is a highly contagious virus that causes yearly epidemics and occasional pandemics of great consequence. Influenza virus-neutralizing antibodies (NAbs) are promising prophylactic and therapeutic reagents. Detection of NAbs in serum samples is critical to evaluate the prevalence and spread of new virus strains. Here we describe the development of a simple, sensitive, specific and safe screening assay for the rapid detection of NAbs against highly pathogenic influenza viruses under BSL-2 conditions. This assay is based on the use of influenza viruses in which the hemagglutinin (HA) gene is replaced by one expressing GFP. These GFP-expressing influenza viruses replicate to high titers in HA-expressing cell lines, but in non-HA expressing cells their replication is restricted to a single cycle.
PMID: 19939917 [PubMed - as supplied by publisher]
-
------