tetano
Editor, Senior Moderator
J Virol
. 2025 Jul 10:e0028325.
doi: 10.1128/jvi.00283-25. Online ahead of print. CX1/BtSY2 and BANAL-20-52 exhibit broader receptor binding and higher affinities to multiple animal ACE2 orthologs than SARS-CoV-2 prototype
Zepeng Xu[SUP] #[/SUP][SUP] 1 2 3 [/SUP], Linjie Li[SUP] #[/SUP][SUP] 2 3 [/SUP], Yuhang Gu[SUP] 4 [/SUP], Dedong Li[SUP] 3 [/SUP], Jianxun Qi[SUP] 2 3 [/SUP], Kefang Liu[SUP] 2 3 [/SUP], Chu-Xia Deng[SUP] 1 [/SUP], George Fu Gao[SUP] 3 [/SUP]
Affiliations
Animal coronaviruses (CoVs) CX1 (formerly named BtSY2) and BANAL-20-52 are phylogenetically closely related to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and CX1 is the first observed animal betaCoV carrying naturally occurring Y501 in its receptor-binding domain (RBD) of the spike (S) protein, a residue related to human adaptation and broader host range. We evaluated the ACE2 usage of CX1 and BANAL-20-52 and observed broader receptor binding spectra and higher affinities to most of the tested animal ACE2 orthologs than the SARS-CoV-2 prototype. Determination of the cryo-EM structures of their S proteins and RBD/hACE2 complexes reveals that Y501 is inter-replaceable with H498 substitution while synergetic with R498 for human ACE2 binding. These results provide further structural insights into SARS-CoV-2 receptor recognition and address the importance of surveillance on potential emerging CoVs.IMPORTANCESince the outbreak of COVID-19, forewarning and prevention of the next pandemic have been widely discussed. Coronaviruses (CoVs) CX1 (formerly named BtSY2) and BANAL-20-52 are phylogenetically closely related to SARS-CoV-2. Particularly, CX1 is the first SARS-CoV-2-related CoV containing Y501 in its receptor-binding domain (RBD) of the spike (S) protein. This study evaluated the interspecies transmission potential of the two CoVs and structurally elucidated the interplay between two RBD residues 498 and 501 on ACE2 binding, further highlighting the importance of surveillance on zoonotic CoVs.
Keywords: BANAL-20-52; CX1; SARS-CoV-2 related coronaviruses; cryo-EM; host range; human ACE2; interspecies transmission; spike.
. 2025 Jul 10:e0028325.
doi: 10.1128/jvi.00283-25. Online ahead of print. CX1/BtSY2 and BANAL-20-52 exhibit broader receptor binding and higher affinities to multiple animal ACE2 orthologs than SARS-CoV-2 prototype
Zepeng Xu[SUP] #[/SUP][SUP] 1 2 3 [/SUP], Linjie Li[SUP] #[/SUP][SUP] 2 3 [/SUP], Yuhang Gu[SUP] 4 [/SUP], Dedong Li[SUP] 3 [/SUP], Jianxun Qi[SUP] 2 3 [/SUP], Kefang Liu[SUP] 2 3 [/SUP], Chu-Xia Deng[SUP] 1 [/SUP], George Fu Gao[SUP] 3 [/SUP]
Affiliations
- PMID: 40637421
- DOI: 10.1128/jvi.00283-25
Animal coronaviruses (CoVs) CX1 (formerly named BtSY2) and BANAL-20-52 are phylogenetically closely related to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and CX1 is the first observed animal betaCoV carrying naturally occurring Y501 in its receptor-binding domain (RBD) of the spike (S) protein, a residue related to human adaptation and broader host range. We evaluated the ACE2 usage of CX1 and BANAL-20-52 and observed broader receptor binding spectra and higher affinities to most of the tested animal ACE2 orthologs than the SARS-CoV-2 prototype. Determination of the cryo-EM structures of their S proteins and RBD/hACE2 complexes reveals that Y501 is inter-replaceable with H498 substitution while synergetic with R498 for human ACE2 binding. These results provide further structural insights into SARS-CoV-2 receptor recognition and address the importance of surveillance on potential emerging CoVs.IMPORTANCESince the outbreak of COVID-19, forewarning and prevention of the next pandemic have been widely discussed. Coronaviruses (CoVs) CX1 (formerly named BtSY2) and BANAL-20-52 are phylogenetically closely related to SARS-CoV-2. Particularly, CX1 is the first SARS-CoV-2-related CoV containing Y501 in its receptor-binding domain (RBD) of the spike (S) protein. This study evaluated the interspecies transmission potential of the two CoVs and structurally elucidated the interplay between two RBD residues 498 and 501 on ACE2 binding, further highlighting the importance of surveillance on zoonotic CoVs.
Keywords: BANAL-20-52; CX1; SARS-CoV-2 related coronaviruses; cryo-EM; host range; human ACE2; interspecies transmission; spike.