tetano
Editor, Senior Moderator
J Virol
. 2024 Jan 5:e0139823.
doi: 10.1128/jvi.01398-23. Online ahead of print. Antibody inhibition of influenza A virus assembly and release
Yuanyuan He[SUP] 1 2 [/SUP], Zijian Guo[SUP] 1 2 [/SUP], Sofie Subiaur[SUP] 1 2 [/SUP], Ananya Benegal[SUP] 1 2 [/SUP], Michael D Vahey[SUP] 1 2 [/SUP]
Affiliations
Antibodies against influenza A virus provide multifaceted protection against infection. Although sensitive and quantitative assays are widely used to measure inhibition of viral attachment and entry, the ability of diverse antibodies to inhibit viral egress is less clear. We address this challenge by developing an imaging-based approach to measure antibody inhibition of virus release across a panel of monoclonal antibodies targeting the influenza A virus surface proteins. Using this approach, we find that inhibition of viral egress is common and can have similar potency to the ability of an antibody to inhibit viral entry. Insights into this understudied aspect of antibody function may help guide the development of improved countermeasures.
Keywords: antibody; computational model; cross-linking; influenza virus; virus assembly; virus egress.
. 2024 Jan 5:e0139823.
doi: 10.1128/jvi.01398-23. Online ahead of print. Antibody inhibition of influenza A virus assembly and release
Yuanyuan He[SUP] 1 2 [/SUP], Zijian Guo[SUP] 1 2 [/SUP], Sofie Subiaur[SUP] 1 2 [/SUP], Ananya Benegal[SUP] 1 2 [/SUP], Michael D Vahey[SUP] 1 2 [/SUP]
Affiliations
- PMID: 38179944
- DOI: 10.1128/jvi.01398-23
Antibodies against influenza A virus provide multifaceted protection against infection. Although sensitive and quantitative assays are widely used to measure inhibition of viral attachment and entry, the ability of diverse antibodies to inhibit viral egress is less clear. We address this challenge by developing an imaging-based approach to measure antibody inhibition of virus release across a panel of monoclonal antibodies targeting the influenza A virus surface proteins. Using this approach, we find that inhibition of viral egress is common and can have similar potency to the ability of an antibody to inhibit viral entry. Insights into this understudied aspect of antibody function may help guide the development of improved countermeasures.
Keywords: antibody; computational model; cross-linking; influenza virus; virus assembly; virus egress.