tetano
Editor, Senior Moderator
J Vet Diagn Invest
. 2022 Jan 17;10406387211068023.
doi: 10.1177/10406387211068023. Online ahead of print.
Review of genome sequencing technologies in molecular characterization of influenza A viruses in swine
Ravendra P Chauhan[SUP] 1 [/SUP], Michelle L Gordon[SUP] 1 [/SUP]
Affiliations
Abstract
The rapidly evolving antigenic diversity of influenza A virus (IAV) genomes in swine makes it imperative to detect emerging novel strains and track their circulation. We analyzed in our review the sequencing technologies used for subtyping and characterizing swine IAV genomes. Google Scholar, PubMed, and International Nucleotide Sequence Database Collaboration (INSDC) database searches identified 216 studies that have utilized Sanger, second-, and third-generation sequencing techniques to subtype and characterize swine IAV genomes up to 31 March 2021. Sanger dideoxy sequencing was by far the most widely used sequencing technique for generating either full-length (43.0%) or partial (31.0%) IAV genomes in swine globally; however, in the last decade, other sequencing platforms such as Illumina have emerged as serious competitors for the generation of whole-genome sequences of swine IAVs. Although partial HA and NA gene sequences were sufficient to determine swine IAV subtypes, whole-genome sequences were critical for determining reassortments and identifying unusual or less frequently occurring IAV subtypes. The combination of Sanger and second-generation sequencing technologies also greatly improved swine IAV characterization. In addition, the rapidly evolving third-generation sequencing platform, MinION, appears promising for on-site, real-time sequencing of complete swine IAV genomes. With a higher raw read accuracy, the use of the MinION could enhance the scalability of swine IAV testing in the field and strengthen the swine IAV disease outbreak response.
Keywords: IAV subtyping; IAV surveillance; MinION, next-generation sequencing; Sanger sequencing; influenza A virus; swine IAV sequencing; whole-genome sequencing.
. 2022 Jan 17;10406387211068023.
doi: 10.1177/10406387211068023. Online ahead of print.
Review of genome sequencing technologies in molecular characterization of influenza A viruses in swine
Ravendra P Chauhan[SUP] 1 [/SUP], Michelle L Gordon[SUP] 1 [/SUP]
Affiliations
- PMID: 35037523
- DOI: 10.1177/10406387211068023
Abstract
The rapidly evolving antigenic diversity of influenza A virus (IAV) genomes in swine makes it imperative to detect emerging novel strains and track their circulation. We analyzed in our review the sequencing technologies used for subtyping and characterizing swine IAV genomes. Google Scholar, PubMed, and International Nucleotide Sequence Database Collaboration (INSDC) database searches identified 216 studies that have utilized Sanger, second-, and third-generation sequencing techniques to subtype and characterize swine IAV genomes up to 31 March 2021. Sanger dideoxy sequencing was by far the most widely used sequencing technique for generating either full-length (43.0%) or partial (31.0%) IAV genomes in swine globally; however, in the last decade, other sequencing platforms such as Illumina have emerged as serious competitors for the generation of whole-genome sequences of swine IAVs. Although partial HA and NA gene sequences were sufficient to determine swine IAV subtypes, whole-genome sequences were critical for determining reassortments and identifying unusual or less frequently occurring IAV subtypes. The combination of Sanger and second-generation sequencing technologies also greatly improved swine IAV characterization. In addition, the rapidly evolving third-generation sequencing platform, MinION, appears promising for on-site, real-time sequencing of complete swine IAV genomes. With a higher raw read accuracy, the use of the MinION could enhance the scalability of swine IAV testing in the field and strengthen the swine IAV disease outbreak response.
Keywords: IAV subtyping; IAV surveillance; MinION, next-generation sequencing; Sanger sequencing; influenza A virus; swine IAV sequencing; whole-genome sequencing.