tetano
Editor, Senior Moderator
J Pediatric Infect Dis Soc
. 2023 May 26;piad033.
doi: 10.1093/jpids/piad033. Online ahead of print. A randomized controlled trial to compare immunogenicity to cell-based vs. live-attenuated influenza vaccines in children
Katherine V Williams[SUP] 1 [/SUP], Zhu-Nan Li[SUP] 2 [/SUP], Bo Zhai[SUP] 3 [/SUP], John F Alcorn[SUP] 3 4 [/SUP], Mary Patricia Nowalk[SUP] 1 [/SUP], Min Z Levine[SUP] 2 [/SUP], Sara S Kim[SUP] 2 [/SUP], Brendan Flannery[SUP] 2 [/SUP], Krissy Moehling Geffel[SUP] 1 [/SUP], Amanda Jaber Merranko[SUP] 5 [/SUP], Mark Collins[SUP] 1 [/SUP], Michael Susick[SUP] 1 [/SUP], Karen S Clarke[SUP] 1 [/SUP], Richard K Zimmerman[SUP] 1 [/SUP], Judith M Martin[SUP] 4 [/SUP]
Affiliations
Background: Few studies have focused on the immune response to more recent influenza vaccine formulations such as cell-cultured inactivated influenza vaccine (ccIIV4) or live attenuated influenza vaccine (LAIV4) in older children and young adults, or differences in immunoglobulin response using newer antibody landscape technology.
Methods: Participants ages 4-21 were randomized to receive ccIIV4 (n =112) or LAIV4 (n=118). A novel high-throughput multiplex influenza antibody detection assay (MIADA) was used to provide detailed IgG, IgA, and IgM antibody isotypes, along with hemagglutination inhibition levels (HAI), measured pre- and 28 days post vaccination.
Results: The HAI and immunoglobulin isotype response to ccIIV4 was greater than LAIV4, with significant increases in IgG but not IgA or IgM. The youngest participants had the highest LAIV4 response. Prior LAIV4 vaccination was associated with a higher response to current season ccIIV4. Cross-reactive A/Delaware/55/2019(H1N1)pdm09 antibodies were present pre-vaccination and increased in response to ccIIV4, but not LAIV4. Immunoglobulin assays strongly correlated with and confirmed the findings of HAI titers to measure immune response.
Conclusions: Age and prior season vaccination may play a role in the immune response in children and young adults to ccIIV4 and LAIV4. While immunoglobulin isotypes provide high level antigen-specific information, HAI titers alone can provide a meaningful representation of day 28 post vaccination response.
Keywords: MIADA; cell-culture-based inactivated influenza vaccine; egg-based live attenuated influenza vaccine; hemagglutination inhibition assay; influenza; influenza vaccine; randomized controlled trial.
. 2023 May 26;piad033.
doi: 10.1093/jpids/piad033. Online ahead of print. A randomized controlled trial to compare immunogenicity to cell-based vs. live-attenuated influenza vaccines in children
Katherine V Williams[SUP] 1 [/SUP], Zhu-Nan Li[SUP] 2 [/SUP], Bo Zhai[SUP] 3 [/SUP], John F Alcorn[SUP] 3 4 [/SUP], Mary Patricia Nowalk[SUP] 1 [/SUP], Min Z Levine[SUP] 2 [/SUP], Sara S Kim[SUP] 2 [/SUP], Brendan Flannery[SUP] 2 [/SUP], Krissy Moehling Geffel[SUP] 1 [/SUP], Amanda Jaber Merranko[SUP] 5 [/SUP], Mark Collins[SUP] 1 [/SUP], Michael Susick[SUP] 1 [/SUP], Karen S Clarke[SUP] 1 [/SUP], Richard K Zimmerman[SUP] 1 [/SUP], Judith M Martin[SUP] 4 [/SUP]
Affiliations
- PMID: 37232430
- DOI: 10.1093/jpids/piad033
Background: Few studies have focused on the immune response to more recent influenza vaccine formulations such as cell-cultured inactivated influenza vaccine (ccIIV4) or live attenuated influenza vaccine (LAIV4) in older children and young adults, or differences in immunoglobulin response using newer antibody landscape technology.
Methods: Participants ages 4-21 were randomized to receive ccIIV4 (n =112) or LAIV4 (n=118). A novel high-throughput multiplex influenza antibody detection assay (MIADA) was used to provide detailed IgG, IgA, and IgM antibody isotypes, along with hemagglutination inhibition levels (HAI), measured pre- and 28 days post vaccination.
Results: The HAI and immunoglobulin isotype response to ccIIV4 was greater than LAIV4, with significant increases in IgG but not IgA or IgM. The youngest participants had the highest LAIV4 response. Prior LAIV4 vaccination was associated with a higher response to current season ccIIV4. Cross-reactive A/Delaware/55/2019(H1N1)pdm09 antibodies were present pre-vaccination and increased in response to ccIIV4, but not LAIV4. Immunoglobulin assays strongly correlated with and confirmed the findings of HAI titers to measure immune response.
Conclusions: Age and prior season vaccination may play a role in the immune response in children and young adults to ccIIV4 and LAIV4. While immunoglobulin isotypes provide high level antigen-specific information, HAI titers alone can provide a meaningful representation of day 28 post vaccination response.
Keywords: MIADA; cell-culture-based inactivated influenza vaccine; egg-based live attenuated influenza vaccine; hemagglutination inhibition assay; influenza; influenza vaccine; randomized controlled trial.