tetano
Editor, Senior Moderator
J Microbiol Immunol Infect
. 2021 Feb 1;S1684-1182(21)00019-0.
doi: 10.1016/j.jmii.2021.01.005. Online ahead of print.
Clinical validation of an automated reverse transcription-insulated isothermal PCR assay for the detection of severe acute respiratory syndrome coronavirus 2
Pai-Ling Chang[SUP] 1 [/SUP], Chia-Yi Lin[SUP] 2 [/SUP], Cheng-Pin Chen[SUP] 3 [/SUP], Yi-Chun Lin[SUP] 4 [/SUP], Hui-Chun Hu[SUP] 5 [/SUP], Shu-Hsing Cheng[SUP] 6 [/SUP], Chien-Yu Cheng[SUP] 7 [/SUP]
Affiliations
Abstract
To determine clinical performance of the single-target SARS-CoV-2 orf 1 ab reverse transcription-insulated isothermal PCR (RT-iiPCR) assay, the positive percentage agreement between this assay and a laboratory real-time RT-PCR assay was 96.8% (30 of 31; 95% confidence interval [CI], 90.5%-100%) and the negative percentage agreement was 97.1% (67 of 69; 95% CI, 93.1%-100%).
Keywords: COVID-19; Insulated isothermal PCR; SARS-CoV-2.
. 2021 Feb 1;S1684-1182(21)00019-0.
doi: 10.1016/j.jmii.2021.01.005. Online ahead of print.
Clinical validation of an automated reverse transcription-insulated isothermal PCR assay for the detection of severe acute respiratory syndrome coronavirus 2
Pai-Ling Chang[SUP] 1 [/SUP], Chia-Yi Lin[SUP] 2 [/SUP], Cheng-Pin Chen[SUP] 3 [/SUP], Yi-Chun Lin[SUP] 4 [/SUP], Hui-Chun Hu[SUP] 5 [/SUP], Shu-Hsing Cheng[SUP] 6 [/SUP], Chien-Yu Cheng[SUP] 7 [/SUP]
Affiliations
- PMID: 33582073
- DOI: 10.1016/j.jmii.2021.01.005
Abstract
To determine clinical performance of the single-target SARS-CoV-2 orf 1 ab reverse transcription-insulated isothermal PCR (RT-iiPCR) assay, the positive percentage agreement between this assay and a laboratory real-time RT-PCR assay was 96.8% (30 of 31; 95% confidence interval [CI], 90.5%-100%) and the negative percentage agreement was 97.1% (67 of 69; 95% CI, 93.1%-100%).
Keywords: COVID-19; Insulated isothermal PCR; SARS-CoV-2.