tetano
Editor, Senior Moderator
J Med Virol
. 2021 Jan 28.
doi: 10.1002/jmv.26823. Online ahead of print.
Will the emergent SARS-CoV2 B.1.1.7 lineage affect molecular diagnosis of COVID19?
Juan David Ram?rez[SUP] 1 [/SUP], Marina Mu?oz[SUP] 1 [/SUP], Luz Helena Pati?o[SUP] 1 [/SUP], Nathalia Ballesteros[SUP] 1 [/SUP], Alberto Paniz-Mondolfi[SUP] 2 3 [/SUP]
Affiliations
Abstract
As the COVID19 pandemic keep tackling global public health systems worldwide. The SARS-CoV2 genome keeps mutating. In that regard, the recent emergence of the B.1.1.7 lineage in the United Kingdom has called the attention of global authorities. One point of concern is that if this lineage can be detected by traditional molecular schemes for SARS-CoV2 detection. Herein, we showed that this lineage does not affect the Berlin-Charit? protocol but can challenge the available commercial kits directed to the Spike (S) gene. All efforts should be made to continue to monitor SARS-CoV-2 genomes for potential variants that can impair diagnostic testing and lead to false negative results. This article is protected by copyright. All rights reserved.
Keywords: Coronavirus; DNA extraction; Genetic variation; Genetics; MethodsVirulenceVirus classification; Pathogenesis; Research and Analysis; otypic variation; ticsPathogenesisPhen.
. 2021 Jan 28.
doi: 10.1002/jmv.26823. Online ahead of print.
Will the emergent SARS-CoV2 B.1.1.7 lineage affect molecular diagnosis of COVID19?
Juan David Ram?rez[SUP] 1 [/SUP], Marina Mu?oz[SUP] 1 [/SUP], Luz Helena Pati?o[SUP] 1 [/SUP], Nathalia Ballesteros[SUP] 1 [/SUP], Alberto Paniz-Mondolfi[SUP] 2 3 [/SUP]
Affiliations
- PMID: 33506970
- DOI: 10.1002/jmv.26823
Abstract
As the COVID19 pandemic keep tackling global public health systems worldwide. The SARS-CoV2 genome keeps mutating. In that regard, the recent emergence of the B.1.1.7 lineage in the United Kingdom has called the attention of global authorities. One point of concern is that if this lineage can be detected by traditional molecular schemes for SARS-CoV2 detection. Herein, we showed that this lineage does not affect the Berlin-Charit? protocol but can challenge the available commercial kits directed to the Spike (S) gene. All efforts should be made to continue to monitor SARS-CoV-2 genomes for potential variants that can impair diagnostic testing and lead to false negative results. This article is protected by copyright. All rights reserved.
Keywords: Coronavirus; DNA extraction; Genetic variation; Genetics; MethodsVirulenceVirus classification; Pathogenesis; Research and Analysis; otypic variation; ticsPathogenesisPhen.