tetano
Editor, Senior Moderator
J Med Virol
. 2022 May 8.
doi: 10.1002/jmv.27843. Online ahead of print.
The Effect of Influenza Virus on The Metabolism of Peripheral Blood Mononuclear Cells with Metabolomics Approach
Zeinab Karimi[SUP] 1 [/SUP], Afsaneh Arefi Oskouie[SUP] 2 [/SUP], Farhad Rezaie[SUP] 1 [/SUP], Fatemeh Ajaminejad[SUP] 1 [/SUP], Sayed Mahdi Marashi[SUP] 1 [/SUP], Talat Mokhtari Azad[SUP] 1 [/SUP]
Affiliations
Abstract
Respiratory viruses led to many deaths and hospitalization per year in the world. Influenza virus is one of the most important respiratory viruses. Recently, the metabolic studies in viral infections have been widely considered by scientists. Metabolomics states the metabolites present in a living organism under certain conditions. In this study, peripheral blood mononuclear cells were spinoculated using virus produced by the Madin-Darby Canine Kidney cell culture system and cells were harvested following spinoculation by influenza virus. Isolation of peripheral blood mononuclear cells was performed by Ficoll-Paque density gradient centrifugation. Metabolites were extracted using organic and Water approaches. Metabolic profiling was performed by a non-targeted using LC-MS/MS technique. Multivariate analysis methods used to determine the main variables. the metabolic pathways involved were determined using databases. Results of present study showed change in biosynthesis pathways such as lipids, polyamines, catecholamines and vitamins. Findings also showed that it is possible to explain the process of inflammation caused by the influenza virus by studying the metabolism of immune cells. This article is protected by copyright. All rights reserved.
Keywords: Influenza virus; LC/MS/MS; Metabolism; Metabolomics; Peripheral Blood Mononuclear Cells.
. 2022 May 8.
doi: 10.1002/jmv.27843. Online ahead of print.
The Effect of Influenza Virus on The Metabolism of Peripheral Blood Mononuclear Cells with Metabolomics Approach
Zeinab Karimi[SUP] 1 [/SUP], Afsaneh Arefi Oskouie[SUP] 2 [/SUP], Farhad Rezaie[SUP] 1 [/SUP], Fatemeh Ajaminejad[SUP] 1 [/SUP], Sayed Mahdi Marashi[SUP] 1 [/SUP], Talat Mokhtari Azad[SUP] 1 [/SUP]
Affiliations
- PMID: 35527001
- DOI: 10.1002/jmv.27843
Abstract
Respiratory viruses led to many deaths and hospitalization per year in the world. Influenza virus is one of the most important respiratory viruses. Recently, the metabolic studies in viral infections have been widely considered by scientists. Metabolomics states the metabolites present in a living organism under certain conditions. In this study, peripheral blood mononuclear cells were spinoculated using virus produced by the Madin-Darby Canine Kidney cell culture system and cells were harvested following spinoculation by influenza virus. Isolation of peripheral blood mononuclear cells was performed by Ficoll-Paque density gradient centrifugation. Metabolites were extracted using organic and Water approaches. Metabolic profiling was performed by a non-targeted using LC-MS/MS technique. Multivariate analysis methods used to determine the main variables. the metabolic pathways involved were determined using databases. Results of present study showed change in biosynthesis pathways such as lipids, polyamines, catecholamines and vitamins. Findings also showed that it is possible to explain the process of inflammation caused by the influenza virus by studying the metabolism of immune cells. This article is protected by copyright. All rights reserved.
Keywords: Influenza virus; LC/MS/MS; Metabolism; Metabolomics; Peripheral Blood Mononuclear Cells.