tetano
Editor, Senior Moderator
J Med Virol
. 2024 Jan;96(1):e29361.
doi: 10.1002/jmv.29361. Saliva-based Proteinase K method: A rapid and reliable diagnostic tool for the detection of SARS-COV-2 in children
Min Jung Kim[SUP] 1 [/SUP], Pil-Gu Park[SUP] 2 [/SUP], Su Jin Hwang[SUP] 3 [/SUP], Seung Jun Bang[SUP] 4 [/SUP], Jae Hwa Jung[SUP] 5 [/SUP], Eun Bin Kown[SUP] 1 [/SUP], Eun Kyung Sul[SUP] 1 [/SUP], Kyung Chul Song[SUP] 1 [/SUP], Joon-Sik Choi[SUP] 1 [/SUP], Kyeo Re Han[SUP] 3 [/SUP], Hye Young Lee[SUP] 6 [/SUP], Dong Hyun Kim[SUP] 4 [/SUP], Jae Myun Lee[SUP] 3 [/SUP]
Affiliations
Early and accurate detection of viruses in children might help prevent transmission and severe diseases. In this study, the severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) detection in children was evaluated using saliva specimens with a Proteinase K (PTK)-based RNA preparation, as saliva collection is a simple and noninvasive procedure, even in young children, with fewer concerns about sample contamination. The saliva-based PTK and the conventional paired nasopharyngeal aspiration (NPA)-based detection methods were compared between COVID-19-positive and -negative children. In addition, the detection rate for SARS-COV-2 and the difference between admission and discharge by the saliva-based PTK method was tested in COVID-19 patients. The diagnostic accuracy of the saliva-based PTK method was 98.8% compared to NP swab-based reverse transcriptase polymerase chain reaction. Saliva samples showed high sensitivity (94.1%) and specificity (100%) when using the PTK method. Furthermore, the saliva-based PTK method significantly reduced the test processing time by 2 h. Notably, C[SUB]t[/SUB] values at discharge increased in saliva samples compared with those at admission, which might indicate patients' clinical conditions or virus activity. In conclusion, the saliva-based PTK implemented in this study streamlines RNA extraction, making the process faster, safer, and more cost-effective, demonstrating that this method is a rapid and reliable diagnostic tool for SARS-CoV-2 detection in children.
Keywords: COVID-19; Proteinase K; SARS-COV-2; children; diagnostics; saliva.
. 2024 Jan;96(1):e29361.
doi: 10.1002/jmv.29361. Saliva-based Proteinase K method: A rapid and reliable diagnostic tool for the detection of SARS-COV-2 in children
Min Jung Kim[SUP] 1 [/SUP], Pil-Gu Park[SUP] 2 [/SUP], Su Jin Hwang[SUP] 3 [/SUP], Seung Jun Bang[SUP] 4 [/SUP], Jae Hwa Jung[SUP] 5 [/SUP], Eun Bin Kown[SUP] 1 [/SUP], Eun Kyung Sul[SUP] 1 [/SUP], Kyung Chul Song[SUP] 1 [/SUP], Joon-Sik Choi[SUP] 1 [/SUP], Kyeo Re Han[SUP] 3 [/SUP], Hye Young Lee[SUP] 6 [/SUP], Dong Hyun Kim[SUP] 4 [/SUP], Jae Myun Lee[SUP] 3 [/SUP]
Affiliations
- PMID: 38178612
- DOI: 10.1002/jmv.29361
Early and accurate detection of viruses in children might help prevent transmission and severe diseases. In this study, the severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) detection in children was evaluated using saliva specimens with a Proteinase K (PTK)-based RNA preparation, as saliva collection is a simple and noninvasive procedure, even in young children, with fewer concerns about sample contamination. The saliva-based PTK and the conventional paired nasopharyngeal aspiration (NPA)-based detection methods were compared between COVID-19-positive and -negative children. In addition, the detection rate for SARS-COV-2 and the difference between admission and discharge by the saliva-based PTK method was tested in COVID-19 patients. The diagnostic accuracy of the saliva-based PTK method was 98.8% compared to NP swab-based reverse transcriptase polymerase chain reaction. Saliva samples showed high sensitivity (94.1%) and specificity (100%) when using the PTK method. Furthermore, the saliva-based PTK method significantly reduced the test processing time by 2 h. Notably, C[SUB]t[/SUB] values at discharge increased in saliva samples compared with those at admission, which might indicate patients' clinical conditions or virus activity. In conclusion, the saliva-based PTK implemented in this study streamlines RNA extraction, making the process faster, safer, and more cost-effective, demonstrating that this method is a rapid and reliable diagnostic tool for SARS-CoV-2 detection in children.
Keywords: COVID-19; Proteinase K; SARS-COV-2; children; diagnostics; saliva.