tetano
Editor, Senior Moderator
J Med Virol
. 2022 Jan 25.
doi: 10.1002/jmv.27617. Online ahead of print.
Recent advances and clinical application in point-of-care testing of SARS-CoV-2
Qing Ye[SUP] 1 [/SUP], Dezhao Lu[SUP] 2 [/SUP], Ting Zhang[SUP] 3 [/SUP], Jianhua Mao[SUP] 1 [/SUP], Shiqiang Shang[SUP] 1 [/SUP]
Affiliations
Abstract
The novel coronavirus 2019 (COVID-19) caused by SARS-CoV-2 spread rapidly worldwide, posing a severe threat to public life and health. It is significant to realize rapid testing and timely control of epidemic situations under the condition of limited resources. However, laboratory-based standardized nucleic acid detection methods have a long turnaround time and high cost, so it is urgent to develop convenient methods for detecting COVID-19. This paper summarizes the point-of-care testing (POCT) developed for novel coronavirus from three aspects: nucleic acid extraction, nucleic acid amplification, and detection methods. This paper introduces a commercial real-time detection system that integrates the abovementioned three steps and the matters needing attention in use. The primary purpose of this review is to provide a reference for emergency response and rapid deployment of COVID-19 and some other emerging infectious diseases. This article is protected by copyright. All rights reserved.
Keywords: COVID-19; instant detection; novel coronavirus; nucleic acid detection.
. 2022 Jan 25.
doi: 10.1002/jmv.27617. Online ahead of print.
Recent advances and clinical application in point-of-care testing of SARS-CoV-2
Qing Ye[SUP] 1 [/SUP], Dezhao Lu[SUP] 2 [/SUP], Ting Zhang[SUP] 3 [/SUP], Jianhua Mao[SUP] 1 [/SUP], Shiqiang Shang[SUP] 1 [/SUP]
Affiliations
- PMID: 35080017
- DOI: 10.1002/jmv.27617
Abstract
The novel coronavirus 2019 (COVID-19) caused by SARS-CoV-2 spread rapidly worldwide, posing a severe threat to public life and health. It is significant to realize rapid testing and timely control of epidemic situations under the condition of limited resources. However, laboratory-based standardized nucleic acid detection methods have a long turnaround time and high cost, so it is urgent to develop convenient methods for detecting COVID-19. This paper summarizes the point-of-care testing (POCT) developed for novel coronavirus from three aspects: nucleic acid extraction, nucleic acid amplification, and detection methods. This paper introduces a commercial real-time detection system that integrates the abovementioned three steps and the matters needing attention in use. The primary purpose of this review is to provide a reference for emergency response and rapid deployment of COVID-19 and some other emerging infectious diseases. This article is protected by copyright. All rights reserved.
Keywords: COVID-19; instant detection; novel coronavirus; nucleic acid detection.