tetano
Editor, Senior Moderator
J Med Virol
. 2021 Jun 25.
doi: 10.1002/jmv.27160. Online ahead of print.
Polymorphisms of ACE (I/D) and ACE2 Receptor Gene (Rs2106809, Rs2285666) are not Related to the Clinical Course of COVID-19; A Case Study
Sevim Karakaş Çelik[SUP] 1 [/SUP], Güneş Çakmak Genç[SUP] 1 [/SUP], Nihal Pişkin[SUP] 2 [/SUP], Bilgehan Açikgöz[SUP] 3 [/SUP], Bülent Altinsoy[SUP] 4 [/SUP], Başak Kurucu Işsiz[SUP] 1 [/SUP], Ahmet Dursun[SUP] 1 [/SUP]
Affiliations
Abstract
Coronavirus disease 2019 (COVID-19) is an infectious disease, that's the reason for the currently ongoing pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Angiotensin-converting enzyme (ACE2) has been recognized as the specific receptor of the SARS-CoV-2 virus. Although the possible effect of ACE2 gene polymorphism remains unknown, human ACE2 receptor expression influences SARS-CoV-2 susceptibility and COVID-19 disease outcome. In this study, we aimed to investigate the relationship between ACE gene I/D polymorphism, ACE2 receptor gene polymorphism, and COVID-19 severity.
Methods: ACE gene insertion/deletion (I/D) polymorphism and ACE2 receptor gene rs2106809 and rs2285666 polymorphisms were determined using polymerase chain reaction (PCR) and PCR-based restriction fragment length polymorphism (RFLP) methods, respectively in 155 COVID-19 patients who were divided into 3 groups (mild, moderate, and severe) according to clinical symptoms.
Results: However, the distribution of genotype and allele frequencies of ACE gene I/D, ACE2 receptor gene rs2106809, and rs2285666 polymorphisms were not statistically significant in all groups.
Conclusions: In conclusion, in the study population, ACE gene I/D, ACE2 receptor gene rs2106809, and rs2285666 polymorphisms were not associated with the severity of COVID-19 infection. Although, ACE2 receptor gene expression may affect the susceptibility to COVID-19, there is no existing evidence that the ACE or ACE2 gene polymorphisms are directly associated with COVID-19 severity. Interindividual differences in COVID-19 severity might be related to epigenetic mechanisms of ACE2 receptor gene expression or variations in other genes suggested to have critical role in COVID-19 pathogenesis such as proinflammatory cytokines, coagulation indicators. This article is protected by copyright. All rights reserved.
Keywords: ACE; ACE2; COVID-19; rs2106809; rs2285666.
. 2021 Jun 25.
doi: 10.1002/jmv.27160. Online ahead of print.
Polymorphisms of ACE (I/D) and ACE2 Receptor Gene (Rs2106809, Rs2285666) are not Related to the Clinical Course of COVID-19; A Case Study
Sevim Karakaş Çelik[SUP] 1 [/SUP], Güneş Çakmak Genç[SUP] 1 [/SUP], Nihal Pişkin[SUP] 2 [/SUP], Bilgehan Açikgöz[SUP] 3 [/SUP], Bülent Altinsoy[SUP] 4 [/SUP], Başak Kurucu Işsiz[SUP] 1 [/SUP], Ahmet Dursun[SUP] 1 [/SUP]
Affiliations
- PMID: 34170561
- DOI: 10.1002/jmv.27160
Abstract
Coronavirus disease 2019 (COVID-19) is an infectious disease, that's the reason for the currently ongoing pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Angiotensin-converting enzyme (ACE2) has been recognized as the specific receptor of the SARS-CoV-2 virus. Although the possible effect of ACE2 gene polymorphism remains unknown, human ACE2 receptor expression influences SARS-CoV-2 susceptibility and COVID-19 disease outcome. In this study, we aimed to investigate the relationship between ACE gene I/D polymorphism, ACE2 receptor gene polymorphism, and COVID-19 severity.
Methods: ACE gene insertion/deletion (I/D) polymorphism and ACE2 receptor gene rs2106809 and rs2285666 polymorphisms were determined using polymerase chain reaction (PCR) and PCR-based restriction fragment length polymorphism (RFLP) methods, respectively in 155 COVID-19 patients who were divided into 3 groups (mild, moderate, and severe) according to clinical symptoms.
Results: However, the distribution of genotype and allele frequencies of ACE gene I/D, ACE2 receptor gene rs2106809, and rs2285666 polymorphisms were not statistically significant in all groups.
Conclusions: In conclusion, in the study population, ACE gene I/D, ACE2 receptor gene rs2106809, and rs2285666 polymorphisms were not associated with the severity of COVID-19 infection. Although, ACE2 receptor gene expression may affect the susceptibility to COVID-19, there is no existing evidence that the ACE or ACE2 gene polymorphisms are directly associated with COVID-19 severity. Interindividual differences in COVID-19 severity might be related to epigenetic mechanisms of ACE2 receptor gene expression or variations in other genes suggested to have critical role in COVID-19 pathogenesis such as proinflammatory cytokines, coagulation indicators. This article is protected by copyright. All rights reserved.
Keywords: ACE; ACE2; COVID-19; rs2106809; rs2285666.