tetano
Editor, Senior Moderator
J Med Virol
. 2024 Sep;96(9):e29871.
doi: 10.1002/jmv.29871. N121T and N121S substitutions on the SARS-CoV-2 spike protein impact on serum neutralization
Van Thi Lo[SUP] 1 [/SUP], Hyun A Lim[SUP] 1 [/SUP], Seong Sik Jang[SUP] 1 [/SUP], Min Chan Kim[SUP] 1 [/SUP], Alain Chrysler Chamfort[SUP] 1 [/SUP], Ha Yeon Kim[SUP] 1 [/SUP], Da Young Mun[SUP] 1 [/SUP], Min Chang Kang[SUP] 1 [/SUP], Han Byul Lee[SUP] 1 [/SUP], Sunjoo Kim[SUP] 2 [/SUP], Younghee Lee[SUP] 1 [/SUP], Sangkyu Park[SUP] 1 [/SUP], Sun-Woo Yoon[SUP] 3 [/SUP], Hye Kwon Kim[SUP] 1 [/SUP]
Affiliations
The N121 site on the spike protein of SARS-CoV-2 is associated with heme and its metabolite, biliverdin, which can affect antibody binding. Both N121T and N121S substitutions have been observed in natural conditions and in a hamster model of dual infection with SARS-CoV-2 and Influenza A virus. Serum pseudotype neutralization assays against HIV-1 particles carrying wild-type, N121T, and N121S spikes with immune mouse and human sera revealed that N121T and N121S mutations had a greater impact on serum neutralization than biliverdin treatment. Although N121T and N121S substitutions are not currently major SARS-CoV-2 variants of concern, this study could provide fundamental information to prepare for potential future mutations at the N121 site of SARS-CoV-2.
Keywords: N121S; N121T; NTD; SARS‐CoV‐2; biliverdin; neutralizing.
. 2024 Sep;96(9):e29871.
doi: 10.1002/jmv.29871. N121T and N121S substitutions on the SARS-CoV-2 spike protein impact on serum neutralization
Van Thi Lo[SUP] 1 [/SUP], Hyun A Lim[SUP] 1 [/SUP], Seong Sik Jang[SUP] 1 [/SUP], Min Chan Kim[SUP] 1 [/SUP], Alain Chrysler Chamfort[SUP] 1 [/SUP], Ha Yeon Kim[SUP] 1 [/SUP], Da Young Mun[SUP] 1 [/SUP], Min Chang Kang[SUP] 1 [/SUP], Han Byul Lee[SUP] 1 [/SUP], Sunjoo Kim[SUP] 2 [/SUP], Younghee Lee[SUP] 1 [/SUP], Sangkyu Park[SUP] 1 [/SUP], Sun-Woo Yoon[SUP] 3 [/SUP], Hye Kwon Kim[SUP] 1 [/SUP]
Affiliations
- PMID: 39221474
- DOI: 10.1002/jmv.29871
The N121 site on the spike protein of SARS-CoV-2 is associated with heme and its metabolite, biliverdin, which can affect antibody binding. Both N121T and N121S substitutions have been observed in natural conditions and in a hamster model of dual infection with SARS-CoV-2 and Influenza A virus. Serum pseudotype neutralization assays against HIV-1 particles carrying wild-type, N121T, and N121S spikes with immune mouse and human sera revealed that N121T and N121S mutations had a greater impact on serum neutralization than biliverdin treatment. Although N121T and N121S substitutions are not currently major SARS-CoV-2 variants of concern, this study could provide fundamental information to prepare for potential future mutations at the N121 site of SARS-CoV-2.
Keywords: N121S; N121T; NTD; SARS‐CoV‐2; biliverdin; neutralizing.