tetano
Editor, Senior Moderator
J Med Virol
. 2021 Jun 1.
doi: 10.1002/jmv.27113. Online ahead of print.
Longitudinal detection of SARS-CoV-2-specific antibody responses with different serological methods
Petra Emmerich[SUP] 1 2 [/SUP], Ronald von Possel[SUP] 1 2 [/SUP], Christoph Josef Hemmer[SUP] 2 [/SUP], Carlos Fritzsche[SUP] 2 [/SUP], Hilte Geerdes-Fenge[SUP] 2 [/SUP], Babett Menge[SUP] 3 [/SUP], Claudia Messing[SUP] 3 [/SUP], Viola Borchardt-Lohölter[SUP] 3 [/SUP], Christina Deschermeier[SUP] 4 [/SUP], Katja Steinhagen[SUP] 3 [/SUP]
Affiliations
Abstract
Serological testing for anti-SARS-CoV-2 antibodies is used to detect ongoing or past SARS-CoV-2 infections. To study the kinetics of anti-SARS-CoV-2 antibodies and to assess the diagnostic performances of eight serological assays, we used 129 serum samples collected on known days post symptom onset (dpso) from 42 patients with PCR-confirmed COVID-19 and 54 serum samples from healthy blood donors, and children infected with seasonal coronaviruses. The sera were analyzed for the presence of IgG, IgM and IgA antibodies using indirect immunofluorescence testing (IIFT) based on SARS-CoV-2-infected cells. They were further tested for antibodies against the S1 domain of the SARS-CoV-2 spike protein (IgG, IgA) and against the viral nucleocapsid protein (IgG, IgM) using ELISAs. The assay specificities were 94.4%-100%. The sensitivities varied largely between assays, reflecting their respective purposes. The sensitivities of IgA and IgM assays were highest between 11 and 20 dpso, whereas the sensitivities of IgG assays peaked between 20 and 60 dpso. IIFT showed highest sensitivities due to the use of the whole SARS-CoV-2 as substrate and provided information on whether or not the individual has been infected with SARS-CoV-2. ELISAs provided further information about both the prevalence and concentration of specific antibodies against selected antigens of SARS-CoV-2. This article is protected by copyright. All rights reserved.
Keywords: COVID-19; ELISA; SARS-CoV-2; antibodies; immunofluorescence; serology.
. 2021 Jun 1.
doi: 10.1002/jmv.27113. Online ahead of print.
Longitudinal detection of SARS-CoV-2-specific antibody responses with different serological methods
Petra Emmerich[SUP] 1 2 [/SUP], Ronald von Possel[SUP] 1 2 [/SUP], Christoph Josef Hemmer[SUP] 2 [/SUP], Carlos Fritzsche[SUP] 2 [/SUP], Hilte Geerdes-Fenge[SUP] 2 [/SUP], Babett Menge[SUP] 3 [/SUP], Claudia Messing[SUP] 3 [/SUP], Viola Borchardt-Lohölter[SUP] 3 [/SUP], Christina Deschermeier[SUP] 4 [/SUP], Katja Steinhagen[SUP] 3 [/SUP]
Affiliations
- PMID: 34061367
- DOI: 10.1002/jmv.27113
Abstract
Serological testing for anti-SARS-CoV-2 antibodies is used to detect ongoing or past SARS-CoV-2 infections. To study the kinetics of anti-SARS-CoV-2 antibodies and to assess the diagnostic performances of eight serological assays, we used 129 serum samples collected on known days post symptom onset (dpso) from 42 patients with PCR-confirmed COVID-19 and 54 serum samples from healthy blood donors, and children infected with seasonal coronaviruses. The sera were analyzed for the presence of IgG, IgM and IgA antibodies using indirect immunofluorescence testing (IIFT) based on SARS-CoV-2-infected cells. They were further tested for antibodies against the S1 domain of the SARS-CoV-2 spike protein (IgG, IgA) and against the viral nucleocapsid protein (IgG, IgM) using ELISAs. The assay specificities were 94.4%-100%. The sensitivities varied largely between assays, reflecting their respective purposes. The sensitivities of IgA and IgM assays were highest between 11 and 20 dpso, whereas the sensitivities of IgG assays peaked between 20 and 60 dpso. IIFT showed highest sensitivities due to the use of the whole SARS-CoV-2 as substrate and provided information on whether or not the individual has been infected with SARS-CoV-2. ELISAs provided further information about both the prevalence and concentration of specific antibodies against selected antigens of SARS-CoV-2. This article is protected by copyright. All rights reserved.
Keywords: COVID-19; ELISA; SARS-CoV-2; antibodies; immunofluorescence; serology.