tetano
Editor, Senior Moderator
J Med Virol
. 2023 Apr;95(4):e28743.
doi: 10.1002/jmv.28743. Immunodominant SARS-CoV-2-specific CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T-cell responses elicited by inactivated vaccines in healthy adults
Jie Ning[SUP] 1 [/SUP], Qinjin Wang[SUP] 1 [/SUP], Ying Chen[SUP] 1 [/SUP], Taojun He[SUP] 1 [/SUP], Fang Zhang[SUP] 1 [/SUP], Xingchi Chen[SUP] 1 [/SUP], Liang Shi[SUP] 1 [/SUP], Aixia Zhai[SUP] 1 [/SUP], Bin Li[SUP] 1 [/SUP], Chao Wu[SUP] 1 [/SUP]
Affiliations
Safety profiles and humoral responses to inactivated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines have been previously assessed, but cellular immune responses to inactivated SARS-CoV-2 vaccines remain understudied. Here, we report the comprehensive characteristics of SARS-CoV-2-specific CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T-cell responses elicited by the BBIBP-CorV vaccine. A total of 295 healthy adults were recruited, and SARS-CoV-2-specific T-cell responses were detected after stimulation with overlapping peptide pools spanning the entire length of the envelope (E), membrane (M), nucleocapsid (N), and spike (S) proteins. Robust and durable CD4[SUP]+[/SUP] (p < 0.0001) and CD8[SUP]+[/SUP] (p < 0.0001) T-cell responses specific to SARS-CoV-2 were detected following the third vaccination, with an increase in specific CD8[SUP]+[/SUP] T-cells, compared to CD4[SUP]+[/SUP] T-cells. Cytokine profiles showed that interferon gamma and tumor necrosis factor-α were predominantly expressed with the negligible expression of interleukin (IL)-4 and IL-10, indicating a Th1- or Tc1-biased response. Compared to E and M proteins, N and S activated a higher proportion of specific T-cells with broader functions. The predominant frequency of the N antigen (49/89) was highest for CD4[SUP]+[/SUP] T-cell immunity. Furthermore, N[SUB]19-36[/SUB] and N[SUB]391-408[/SUB] were identified to contain dominant CD8[SUP]+[/SUP] and CD4[SUP]+[/SUP] T-cell epitopes, respectively. In addition, N[SUB]19-36[/SUB] -specific CD8[SUP]+[/SUP] T-cells were mainly effector memory CD45RA cells, whereas N[SUB]391-408[/SUB] -specific CD4[SUP]+[/SUP] T-cells were mainly effector memory cells. Therefore, this study reports comprehensive features of T-cell immunity induced by the inactivated SARS-CoV-2 vaccine BBIBP-CorV and proposes highly conserved candidate peptides which may be beneficial in vaccine optimization.
Keywords: BBIBP-CorV; SARS-CoV-2; cellular immunity; inactivated vaccine.
. 2023 Apr;95(4):e28743.
doi: 10.1002/jmv.28743. Immunodominant SARS-CoV-2-specific CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T-cell responses elicited by inactivated vaccines in healthy adults
Jie Ning[SUP] 1 [/SUP], Qinjin Wang[SUP] 1 [/SUP], Ying Chen[SUP] 1 [/SUP], Taojun He[SUP] 1 [/SUP], Fang Zhang[SUP] 1 [/SUP], Xingchi Chen[SUP] 1 [/SUP], Liang Shi[SUP] 1 [/SUP], Aixia Zhai[SUP] 1 [/SUP], Bin Li[SUP] 1 [/SUP], Chao Wu[SUP] 1 [/SUP]
Affiliations
- PMID: 37185843
- DOI: 10.1002/jmv.28743
Safety profiles and humoral responses to inactivated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines have been previously assessed, but cellular immune responses to inactivated SARS-CoV-2 vaccines remain understudied. Here, we report the comprehensive characteristics of SARS-CoV-2-specific CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T-cell responses elicited by the BBIBP-CorV vaccine. A total of 295 healthy adults were recruited, and SARS-CoV-2-specific T-cell responses were detected after stimulation with overlapping peptide pools spanning the entire length of the envelope (E), membrane (M), nucleocapsid (N), and spike (S) proteins. Robust and durable CD4[SUP]+[/SUP] (p < 0.0001) and CD8[SUP]+[/SUP] (p < 0.0001) T-cell responses specific to SARS-CoV-2 were detected following the third vaccination, with an increase in specific CD8[SUP]+[/SUP] T-cells, compared to CD4[SUP]+[/SUP] T-cells. Cytokine profiles showed that interferon gamma and tumor necrosis factor-α were predominantly expressed with the negligible expression of interleukin (IL)-4 and IL-10, indicating a Th1- or Tc1-biased response. Compared to E and M proteins, N and S activated a higher proportion of specific T-cells with broader functions. The predominant frequency of the N antigen (49/89) was highest for CD4[SUP]+[/SUP] T-cell immunity. Furthermore, N[SUB]19-36[/SUB] and N[SUB]391-408[/SUB] were identified to contain dominant CD8[SUP]+[/SUP] and CD4[SUP]+[/SUP] T-cell epitopes, respectively. In addition, N[SUB]19-36[/SUB] -specific CD8[SUP]+[/SUP] T-cells were mainly effector memory CD45RA cells, whereas N[SUB]391-408[/SUB] -specific CD4[SUP]+[/SUP] T-cells were mainly effector memory cells. Therefore, this study reports comprehensive features of T-cell immunity induced by the inactivated SARS-CoV-2 vaccine BBIBP-CorV and proposes highly conserved candidate peptides which may be beneficial in vaccine optimization.
Keywords: BBIBP-CorV; SARS-CoV-2; cellular immunity; inactivated vaccine.