tetano
Editor, Senior Moderator
J Med Virol
. 2022 Feb 16.
doi: 10.1002/jmv.27660. Online ahead of print.
Food for thought: Eating before saliva collection and interference with SARS-CoV-2 detection
Matthew M Hernandez[SUP] 1 [/SUP], Mariawy Riollano-Cruz[SUP] 2 [/SUP], Mary C Boyle[SUP] 2 [/SUP], Radhika Banu[SUP] 1 [/SUP], Paras Shrestha[SUP] 1 [/SUP], Brandon Gray[SUP] 1 [/SUP], Liyong Cao[SUP] 1 [/SUP], Feng Chen[SUP] 1 [/SUP], Huanzhi Shi[SUP] 1 [/SUP], Daniel E Paniz-Perez[SUP] 3 [/SUP], Paul A Paniz-Perez[SUP] 3 [/SUP], Aryan L Rishi[SUP] 3 [/SUP], Jacob Dubinsky[SUP] 3 [/SUP], Dylan Dubinsky[SUP] 3 [/SUP], Owen Dubinsky[SUP] 3 [/SUP], Sophie Baine[SUP] 3 [/SUP], Lily Baine[SUP] 3 [/SUP], Suzanne Arinsburg[SUP] 1 [/SUP], Ian Baine[SUP] 1 [/SUP], Juan David Ramirez[SUP] 1 [/SUP], Carlos Cordon-Cardo[SUP] 1 [/SUP], Emilia Mia Sordillo[SUP] 1 [/SUP], Alberto E Paniz-Mondolfi[SUP] 1 [/SUP]
Affiliations
Abstract
Saliva is a promising specimen for detection of viruses that cause upper respiratory infections including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) due to its cost-effectiveness and non-invasive collection. However, together with intrinsic enzymes and oral microbiota, children's unique dietary habits may introduce substances that interfere with diagnostic testing. To determine whether children's dietary choices impact SARS-CoV-2 molecular detection in saliva, we performed a diagnostic study that simulates testing of real-life specimens provided from healthy children (n=5) who self-collected saliva at home before and at 0, 20, and 60 minutes after eating 20 foods they selected. Each of seventy-two specimens was split into two volumes and spiked with SARS-CoV-2-negative or -positive clinical standards prior to side-by-side testing by reverse-transcription polymerase chain reaction matrix-assisted laser desorption ionization time-of-flight (RT-PCR/MALDI-TOF) assay. Detection of internal extraction control and SARS-CoV-2 nucleic acids was reduced in replicates of saliva collected at 0 minutes after eating 11 of 20 foods. Interference resolved at 20 and 60 minutes after eating all foods except hot dog in one participant. This represented a significant improvement in detection of nucleic acids compared to saliva collected at 0 minutes after eating (P=0.0005). We demonstrate successful detection of viral nucleic acids in saliva self-collected by children before and after eating a variety of foods. Fasting is not required before saliva collection for SARS-CoV-2 testing by RT-PCR/MALDI-TOF, but waiting 20 minutes after eating is sufficient for accurate testing. These findings should be considered for SARS-CoV-2 testing and broader viral diagnostics in saliva specimens. This article is protected by copyright. All rights reserved.
Keywords: Interference; MALDI-TOF; RT-PCR; SARS-CoV-2; Saliva.
. 2022 Feb 16.
doi: 10.1002/jmv.27660. Online ahead of print.
Food for thought: Eating before saliva collection and interference with SARS-CoV-2 detection
Matthew M Hernandez[SUP] 1 [/SUP], Mariawy Riollano-Cruz[SUP] 2 [/SUP], Mary C Boyle[SUP] 2 [/SUP], Radhika Banu[SUP] 1 [/SUP], Paras Shrestha[SUP] 1 [/SUP], Brandon Gray[SUP] 1 [/SUP], Liyong Cao[SUP] 1 [/SUP], Feng Chen[SUP] 1 [/SUP], Huanzhi Shi[SUP] 1 [/SUP], Daniel E Paniz-Perez[SUP] 3 [/SUP], Paul A Paniz-Perez[SUP] 3 [/SUP], Aryan L Rishi[SUP] 3 [/SUP], Jacob Dubinsky[SUP] 3 [/SUP], Dylan Dubinsky[SUP] 3 [/SUP], Owen Dubinsky[SUP] 3 [/SUP], Sophie Baine[SUP] 3 [/SUP], Lily Baine[SUP] 3 [/SUP], Suzanne Arinsburg[SUP] 1 [/SUP], Ian Baine[SUP] 1 [/SUP], Juan David Ramirez[SUP] 1 [/SUP], Carlos Cordon-Cardo[SUP] 1 [/SUP], Emilia Mia Sordillo[SUP] 1 [/SUP], Alberto E Paniz-Mondolfi[SUP] 1 [/SUP]
Affiliations
- PMID: 35171508
- DOI: 10.1002/jmv.27660
Abstract
Saliva is a promising specimen for detection of viruses that cause upper respiratory infections including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) due to its cost-effectiveness and non-invasive collection. However, together with intrinsic enzymes and oral microbiota, children's unique dietary habits may introduce substances that interfere with diagnostic testing. To determine whether children's dietary choices impact SARS-CoV-2 molecular detection in saliva, we performed a diagnostic study that simulates testing of real-life specimens provided from healthy children (n=5) who self-collected saliva at home before and at 0, 20, and 60 minutes after eating 20 foods they selected. Each of seventy-two specimens was split into two volumes and spiked with SARS-CoV-2-negative or -positive clinical standards prior to side-by-side testing by reverse-transcription polymerase chain reaction matrix-assisted laser desorption ionization time-of-flight (RT-PCR/MALDI-TOF) assay. Detection of internal extraction control and SARS-CoV-2 nucleic acids was reduced in replicates of saliva collected at 0 minutes after eating 11 of 20 foods. Interference resolved at 20 and 60 minutes after eating all foods except hot dog in one participant. This represented a significant improvement in detection of nucleic acids compared to saliva collected at 0 minutes after eating (P=0.0005). We demonstrate successful detection of viral nucleic acids in saliva self-collected by children before and after eating a variety of foods. Fasting is not required before saliva collection for SARS-CoV-2 testing by RT-PCR/MALDI-TOF, but waiting 20 minutes after eating is sufficient for accurate testing. These findings should be considered for SARS-CoV-2 testing and broader viral diagnostics in saliva specimens. This article is protected by copyright. All rights reserved.
Keywords: Interference; MALDI-TOF; RT-PCR; SARS-CoV-2; Saliva.