tetano
Editor, Senior Moderator
J Med Virol
. 2023 Apr;95(4):e28727.
doi: 10.1002/jmv.28727. Dysbiosis of oropharyngeal microbiome and antibiotic resistance in hospitalized COVID-19 patients
Jiani Wu[SUP] 1 2 3 [/SUP], Wei Liu[SUP] 4 [/SUP], Lin Zhu[SUP] 1 2 [/SUP], Nina Li[SUP] 1 2 [/SUP], Gengyan Luo[SUP] 5 [/SUP], Ming Gu[SUP] 1 2 [/SUP], Minwu Peng[SUP] 5 [/SUP], Shike Zeng[SUP] 1 2 [/SUP], Shu Wu[SUP] 1 2 [/SUP], Shengze Zhang[SUP] 1 2 [/SUP], Qiqi Chen[SUP] 1 2 [/SUP], Meiqi Cai[SUP] 1 2 [/SUP], Wei Cao[SUP] 1 2 [/SUP], Ying Jiang[SUP] 6 [/SUP], Chuming Luo[SUP] 1 2 [/SUP], Dechao Tian[SUP] 1 2 [/SUP], Mang Shi[SUP] 5 [/SUP], Yuelong Shu[SUP] 1 2 7 [/SUP], Guohui Chang[SUP] 4 [/SUP], Huanle Luo[SUP] 1 2 8 [/SUP]
Affiliations
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is ongoing and multiple studies have elucidated its pathogenesis, however, the related- microbiome imbalance caused by SARS-CoV-2 is still not clear. In this study, we have comprehensively compared the microbiome composition and associated function alterations in the oropharyngeal swabs of healthy controls and coronavirus disease 2019 (COVID-19) patients with moderate or severe symptoms by metatranscriptomic sequencing. We did observe a reduced microbiome alpha-diversity but significant enrichment of opportunistic microorganisms in patients with COVID-19 compared with healthy controls, and the microbial homeostasis was rebuilt following the recovery of COVID-19 patients. Correspondingly, less functional genes in multiple biological processes and weakened metabolic pathways such as carbohydrate metabolism, energy metabolism were also observed in COVID-19 patients. We only found higher relative abundance of limited genera such as Lachnoanaerobaculum between severe patients and moderate patients while no worthy-noting microbiome diversity and function alteration were observed. Finally, we noticed that the co-occurrence of antibiotic resistance and virulence was closely related to the microbiome alteration caused by SRAS-CoV-2. Overall, our findings demonstrate that microbial dysbiosis may enhance the pathogenesis of SARS-CoV-2 and the antibiotics treatment should be critically considered.
Keywords: COVID-19; antibiotic resistance; microbiome dysbiosis; virulence.
. 2023 Apr;95(4):e28727.
doi: 10.1002/jmv.28727. Dysbiosis of oropharyngeal microbiome and antibiotic resistance in hospitalized COVID-19 patients
Jiani Wu[SUP] 1 2 3 [/SUP], Wei Liu[SUP] 4 [/SUP], Lin Zhu[SUP] 1 2 [/SUP], Nina Li[SUP] 1 2 [/SUP], Gengyan Luo[SUP] 5 [/SUP], Ming Gu[SUP] 1 2 [/SUP], Minwu Peng[SUP] 5 [/SUP], Shike Zeng[SUP] 1 2 [/SUP], Shu Wu[SUP] 1 2 [/SUP], Shengze Zhang[SUP] 1 2 [/SUP], Qiqi Chen[SUP] 1 2 [/SUP], Meiqi Cai[SUP] 1 2 [/SUP], Wei Cao[SUP] 1 2 [/SUP], Ying Jiang[SUP] 6 [/SUP], Chuming Luo[SUP] 1 2 [/SUP], Dechao Tian[SUP] 1 2 [/SUP], Mang Shi[SUP] 5 [/SUP], Yuelong Shu[SUP] 1 2 7 [/SUP], Guohui Chang[SUP] 4 [/SUP], Huanle Luo[SUP] 1 2 8 [/SUP]
Affiliations
- PMID: 37185870
- DOI: 10.1002/jmv.28727
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is ongoing and multiple studies have elucidated its pathogenesis, however, the related- microbiome imbalance caused by SARS-CoV-2 is still not clear. In this study, we have comprehensively compared the microbiome composition and associated function alterations in the oropharyngeal swabs of healthy controls and coronavirus disease 2019 (COVID-19) patients with moderate or severe symptoms by metatranscriptomic sequencing. We did observe a reduced microbiome alpha-diversity but significant enrichment of opportunistic microorganisms in patients with COVID-19 compared with healthy controls, and the microbial homeostasis was rebuilt following the recovery of COVID-19 patients. Correspondingly, less functional genes in multiple biological processes and weakened metabolic pathways such as carbohydrate metabolism, energy metabolism were also observed in COVID-19 patients. We only found higher relative abundance of limited genera such as Lachnoanaerobaculum between severe patients and moderate patients while no worthy-noting microbiome diversity and function alteration were observed. Finally, we noticed that the co-occurrence of antibiotic resistance and virulence was closely related to the microbiome alteration caused by SRAS-CoV-2. Overall, our findings demonstrate that microbial dysbiosis may enhance the pathogenesis of SARS-CoV-2 and the antibiotics treatment should be critically considered.
Keywords: COVID-19; antibiotic resistance; microbiome dysbiosis; virulence.