tetano
Editor, Senior Moderator
J Med Virol
. 2024 Mar;96(3):e29545.
doi: 10.1002/jmv.29545. Detection of SARS-CoV-2 virus in middle ear effusions and its association with otitis media with effusion
Xiao Fu[SUP] 1 2 3 [/SUP], Zhujian Wang[SUP] 4 [/SUP], Binjun Chen[SUP] 1 2 3 [/SUP], Haojie Sun[SUP] 1 2 3 [/SUP], Jihan Lyu[SUP] 1 2 3 [/SUP], Jing Shao[SUP] 5 [/SUP], Xiaoling Lu[SUP] 1 2 3 [/SUP], Jianghong Xu[SUP] 1 2 3 [/SUP], Juanmei Yang[SUP] 1 2 3 [/SUP], Fanglu Chi[SUP] 1 2 3 [/SUP], Yibo Huang[SUP] 1 2 3 [/SUP], Dongdong Ren[SUP] 1 2 3 [/SUP]
Affiliations
A large-scale outbreak of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) occurred in Shanghai, China, in early December 2022. To study the incidence and characteristics of otitis media with effusion (OME) complicating SARS-CoV-2, we collected 267 middle ear effusion (MEE) samples and 172 nasopharyngeal (NP) swabs from patients. The SARS-CoV-2 virus was detected by RT-PCR targeting. The SARS-CoV-2 virus, angiotensin-converting enzyme 2 (ACE2), and transmembrane serine protease 2 (TMPRSS2) expression in human samples was examined via immunofluorescence. During the COVID-19 epidemic in 2022, the incidence of OME (3%) significantly increased compared to the same period from 2020 to 2022. Ear symptoms in patients with SARS-CoV-2 complicated by OME generally appeared late, even after a negative NP swab, an average of 9.33 ± 6.272 days after COVID-19 infection. The SARS-CoV-2 virus was detected in MEE, which had a higher viral load than NP swabs. The insertion rate of tympanostomy tubes was not significantly higher than in OME patients in 2019-2022. Virus migration led to high viral loads in MEE despite negative NP swabs, indicating that OME lagged behind respiratory infections but had a favorable prognosis. Furthermore, middle ear tissue from adult humans coexpressed the ACE2 receptor for the SARS-CoV-2 virus and the TMPRSS2 cofactors required for virus entry.
Keywords: biostatistics & bioinformatics, RNA extraction; biostatistics & bioinformatics, data processing; coronavirus; research and analysis methods; virus classification, data acquisition.
. 2024 Mar;96(3):e29545.
doi: 10.1002/jmv.29545. Detection of SARS-CoV-2 virus in middle ear effusions and its association with otitis media with effusion
Xiao Fu[SUP] 1 2 3 [/SUP], Zhujian Wang[SUP] 4 [/SUP], Binjun Chen[SUP] 1 2 3 [/SUP], Haojie Sun[SUP] 1 2 3 [/SUP], Jihan Lyu[SUP] 1 2 3 [/SUP], Jing Shao[SUP] 5 [/SUP], Xiaoling Lu[SUP] 1 2 3 [/SUP], Jianghong Xu[SUP] 1 2 3 [/SUP], Juanmei Yang[SUP] 1 2 3 [/SUP], Fanglu Chi[SUP] 1 2 3 [/SUP], Yibo Huang[SUP] 1 2 3 [/SUP], Dongdong Ren[SUP] 1 2 3 [/SUP]
Affiliations
- PMID: 38506248
- DOI: 10.1002/jmv.29545
A large-scale outbreak of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) occurred in Shanghai, China, in early December 2022. To study the incidence and characteristics of otitis media with effusion (OME) complicating SARS-CoV-2, we collected 267 middle ear effusion (MEE) samples and 172 nasopharyngeal (NP) swabs from patients. The SARS-CoV-2 virus was detected by RT-PCR targeting. The SARS-CoV-2 virus, angiotensin-converting enzyme 2 (ACE2), and transmembrane serine protease 2 (TMPRSS2) expression in human samples was examined via immunofluorescence. During the COVID-19 epidemic in 2022, the incidence of OME (3%) significantly increased compared to the same period from 2020 to 2022. Ear symptoms in patients with SARS-CoV-2 complicated by OME generally appeared late, even after a negative NP swab, an average of 9.33 ± 6.272 days after COVID-19 infection. The SARS-CoV-2 virus was detected in MEE, which had a higher viral load than NP swabs. The insertion rate of tympanostomy tubes was not significantly higher than in OME patients in 2019-2022. Virus migration led to high viral loads in MEE despite negative NP swabs, indicating that OME lagged behind respiratory infections but had a favorable prognosis. Furthermore, middle ear tissue from adult humans coexpressed the ACE2 receptor for the SARS-CoV-2 virus and the TMPRSS2 cofactors required for virus entry.
Keywords: biostatistics & bioinformatics, RNA extraction; biostatistics & bioinformatics, data processing; coronavirus; research and analysis methods; virus classification, data acquisition.