tetano
Editor, Senior Moderator
J Med Virol
. 2022 Nov 18.
doi: 10.1002/jmv.28323. Online ahead of print.
Characterisation and analysis of linear epitopes corresponding to SARS-CoV-2 outbreak in Jilin Province, China
Wenying Gao 1 2 , Zhaolong Li 2 , Qingtian Guan 1 , Wenzhe Cui 3 , Baishong Zheng 2 , Qiang Ding 4 , Guoyue Lv 5 , Jiancheng Xu 6 , Wenyan Zhang 1 2
Affiliations
PMID: 36401153 DOI: 10.1002/jmv.28323
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants have caused hundreds of thousands of deaths and shown serious social influence worldwide. Jilin Province, China, experienced the first wave of the outbreak from December 2020 to February 2021. Here, we analysed the genomic characteristics of the SARS-CoV-2 outbreak in Jilin province using a phylogeographic tree and found that clinical isolates belonged to the B.1 lineage, which was considered to be the ancestral lineage. Several dominant SARS-CoV-2 specific linear B cell epitopes that reacted with the convalescent sera were also analysed and identified using a peptide microarray composed of S, M, and E proteins. Moreover, the serum of convalescent patients infected with SARS-CoV-2 showed neutralising activity against four widely spreading SARS-CoV-2 variants; however, significant differences were observed in neutralising activities against different SARS-CoV-2 variants. These data provide important information on genomic characteristics, linear epitopes, and neutralising activity of SARS-CoV-2 outbreak in Jilin Province, China, which may aid in understanding disease patterns and regional aspects of the pandemic. This article is protected by copyright. All rights reserved.
Keywords: B cell epitopes; Neutralizing activity; SARS-CoV-2; convalescent serum; peptide microarray.
. 2022 Nov 18.
doi: 10.1002/jmv.28323. Online ahead of print.
Characterisation and analysis of linear epitopes corresponding to SARS-CoV-2 outbreak in Jilin Province, China
Wenying Gao 1 2 , Zhaolong Li 2 , Qingtian Guan 1 , Wenzhe Cui 3 , Baishong Zheng 2 , Qiang Ding 4 , Guoyue Lv 5 , Jiancheng Xu 6 , Wenyan Zhang 1 2
Affiliations
PMID: 36401153 DOI: 10.1002/jmv.28323
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants have caused hundreds of thousands of deaths and shown serious social influence worldwide. Jilin Province, China, experienced the first wave of the outbreak from December 2020 to February 2021. Here, we analysed the genomic characteristics of the SARS-CoV-2 outbreak in Jilin province using a phylogeographic tree and found that clinical isolates belonged to the B.1 lineage, which was considered to be the ancestral lineage. Several dominant SARS-CoV-2 specific linear B cell epitopes that reacted with the convalescent sera were also analysed and identified using a peptide microarray composed of S, M, and E proteins. Moreover, the serum of convalescent patients infected with SARS-CoV-2 showed neutralising activity against four widely spreading SARS-CoV-2 variants; however, significant differences were observed in neutralising activities against different SARS-CoV-2 variants. These data provide important information on genomic characteristics, linear epitopes, and neutralising activity of SARS-CoV-2 outbreak in Jilin Province, China, which may aid in understanding disease patterns and regional aspects of the pandemic. This article is protected by copyright. All rights reserved.
Keywords: B cell epitopes; Neutralizing activity; SARS-CoV-2; convalescent serum; peptide microarray.