tetano
Editor, Senior Moderator
J Med Virol
. 2024 Mar;96(3):e29454.
doi: 10.1002/jmv.29454. A superior heterologous prime-boost vaccination strategy against COVID-19: A bivalent vaccine based on yeast-derived RBD proteins followed by a heterologous vaccine
Yu Liu[SUP] 1 [/SUP], Miao Li[SUP] 2 [/SUP], Tingting Cui[SUP] 3 [/SUP], Zhian Chen[SUP] 1 [/SUP], Liangting Xu[SUP] 1 [/SUP], Wenjuan Li[SUP] 2 [/SUP], Qinhua Peng[SUP] 2 [/SUP], Xingxing Li[SUP] 2 [/SUP], Danhua Zhao[SUP] 2 [/SUP], C Alexander Valencia[SUP] 1 [/SUP], Biao Dong[SUP] 1 4 [/SUP], Zhongfang Wang[SUP] 3 [/SUP], Hoi Yee Chow[SUP] 1 [/SUP], Yuhua Li[SUP] 2 [/SUP]
Affiliations
Various vaccines have been challenged by SARS-CoV-2 variants. Here, we reported a yeast-derived recombinant bivalent vaccine (Bivalent wild-type [Wt]+De) based on the wt and Delta receptor-binding domain (RBD). Yeast derived RBD proteins based on the wt and Delta mutant were used as the prime vaccine. It was found that, in the presence of aluminium hydroxide (Alum) and unmethylated CpG-oligodeoxynucleotides (CpG) adjuvants, more cross-protective immunity against SARS-CoV-2 prototype and variants were elicited by bivalent vaccine than monovalent wtRBD or Delta RBD. Furthermore, a heterologous boosting strategy consisting of two doses of bivalent vaccines followed by one dose adenovirus vectored vaccine exhibited cross-neutralization capacity and specific T cell responses against Delta and Omicron (BA.1 and BA.4/5) variants in mice, superior to a homologous vaccination strategy. This study suggested that heterologous prime-boost vaccination with yeast-derived bivalent protein vaccine could be a potential approach to address the challenge of emerging variants.
Keywords: Pichia pastoris; SARS-CoV-2; bivalent; heterologous; vaccine.
. 2024 Mar;96(3):e29454.
doi: 10.1002/jmv.29454. A superior heterologous prime-boost vaccination strategy against COVID-19: A bivalent vaccine based on yeast-derived RBD proteins followed by a heterologous vaccine
Yu Liu[SUP] 1 [/SUP], Miao Li[SUP] 2 [/SUP], Tingting Cui[SUP] 3 [/SUP], Zhian Chen[SUP] 1 [/SUP], Liangting Xu[SUP] 1 [/SUP], Wenjuan Li[SUP] 2 [/SUP], Qinhua Peng[SUP] 2 [/SUP], Xingxing Li[SUP] 2 [/SUP], Danhua Zhao[SUP] 2 [/SUP], C Alexander Valencia[SUP] 1 [/SUP], Biao Dong[SUP] 1 4 [/SUP], Zhongfang Wang[SUP] 3 [/SUP], Hoi Yee Chow[SUP] 1 [/SUP], Yuhua Li[SUP] 2 [/SUP]
Affiliations
- PMID: 38445768
- DOI: 10.1002/jmv.29454
Various vaccines have been challenged by SARS-CoV-2 variants. Here, we reported a yeast-derived recombinant bivalent vaccine (Bivalent wild-type [Wt]+De) based on the wt and Delta receptor-binding domain (RBD). Yeast derived RBD proteins based on the wt and Delta mutant were used as the prime vaccine. It was found that, in the presence of aluminium hydroxide (Alum) and unmethylated CpG-oligodeoxynucleotides (CpG) adjuvants, more cross-protective immunity against SARS-CoV-2 prototype and variants were elicited by bivalent vaccine than monovalent wtRBD or Delta RBD. Furthermore, a heterologous boosting strategy consisting of two doses of bivalent vaccines followed by one dose adenovirus vectored vaccine exhibited cross-neutralization capacity and specific T cell responses against Delta and Omicron (BA.1 and BA.4/5) variants in mice, superior to a homologous vaccination strategy. This study suggested that heterologous prime-boost vaccination with yeast-derived bivalent protein vaccine could be a potential approach to address the challenge of emerging variants.
Keywords: Pichia pastoris; SARS-CoV-2; bivalent; heterologous; vaccine.