tetano
Editor, Senior Moderator
J Med Life
. Jul-Aug 2021;14(4):431-442.
doi: 10.25122/jml-2021-0168.
Rapid diagnostic methods for SARS-CoV-2 (COVID-19) detection: an evidence-based report
Manish Kumar Verma[SUP] 1 [/SUP], Parshant Kumar Sharma[SUP] 2 [/SUP], Henu Kumar Verma[SUP] 3 [/SUP], Anand Narayan Singh[SUP] 1 [/SUP], Desh Deepak Singh[SUP] 4 [/SUP], Poonam Verma[SUP] 5 [/SUP], Areena Hoda Siddiqui[SUP] 6 [/SUP]
Affiliations
Abstract
Since December 2019, the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been a global health concern. The transmission method is human-to-human. Since this second wave of SARS-CoV-2 is more aggressive than the first wave, rapid testing is warranted to use practical diagnostics to break the transfer chain. Currently, various techniques are used to diagnose SARS-CoV-2 infection, each with its own set of advantages and disadvantages. A full review of online databases such as PubMed, EMBASE, Web of Science, and Google Scholar was analyzed to identify relevant articles focusing on SARS-CoV-2 and diagnosis and therapeutics. The most recent article search was on May 10, 2021. We summarize promising methods for detecting the novel Coronavirus using sensor-based diagnostic technologies that are sensitive, cost-effective, and simple to use at the point of care. This includes loop-mediated isothermal amplification and several laboratory protocols for confirming suspected 2019-nCoV cases, as well as studies with non-commercial laboratory protocols based on real-time reverse transcription-polymerase chain reaction and a field-effect transistor-based bio-sensing device. We discuss a potential discovery that could lead to the mass and targeted SARS-CoV-2 detection needed to manage the COVID-19 pandemic through infection succession and timely therapy.
Keywords: COVID-19; Point-of-Care Testing; SARS-CoV-2; diagnostics; sensor chip-based.
. Jul-Aug 2021;14(4):431-442.
doi: 10.25122/jml-2021-0168.
Rapid diagnostic methods for SARS-CoV-2 (COVID-19) detection: an evidence-based report
Manish Kumar Verma[SUP] 1 [/SUP], Parshant Kumar Sharma[SUP] 2 [/SUP], Henu Kumar Verma[SUP] 3 [/SUP], Anand Narayan Singh[SUP] 1 [/SUP], Desh Deepak Singh[SUP] 4 [/SUP], Poonam Verma[SUP] 5 [/SUP], Areena Hoda Siddiqui[SUP] 6 [/SUP]
Affiliations
- PMID: 34621365
- PMCID: PMC8485368
- DOI: 10.25122/jml-2021-0168
Abstract
Since December 2019, the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been a global health concern. The transmission method is human-to-human. Since this second wave of SARS-CoV-2 is more aggressive than the first wave, rapid testing is warranted to use practical diagnostics to break the transfer chain. Currently, various techniques are used to diagnose SARS-CoV-2 infection, each with its own set of advantages and disadvantages. A full review of online databases such as PubMed, EMBASE, Web of Science, and Google Scholar was analyzed to identify relevant articles focusing on SARS-CoV-2 and diagnosis and therapeutics. The most recent article search was on May 10, 2021. We summarize promising methods for detecting the novel Coronavirus using sensor-based diagnostic technologies that are sensitive, cost-effective, and simple to use at the point of care. This includes loop-mediated isothermal amplification and several laboratory protocols for confirming suspected 2019-nCoV cases, as well as studies with non-commercial laboratory protocols based on real-time reverse transcription-polymerase chain reaction and a field-effect transistor-based bio-sensing device. We discuss a potential discovery that could lead to the mass and targeted SARS-CoV-2 detection needed to manage the COVID-19 pandemic through infection succession and timely therapy.
Keywords: COVID-19; Point-of-Care Testing; SARS-CoV-2; diagnostics; sensor chip-based.