tetano
Editor, Senior Moderator
J Korean Med Sci. 2020 Feb 24;35(7):e84. doi: 10.3346/jkms.2020.35.e84. [h=1]Virus Isolation from the First Patient with SARS-CoV-2 in Korea.[/h]
Park WB[SUP]#[/SUP][SUP]1,[/SUP][SUP]2[/SUP], Kwon NJ[SUP]#[/SUP][SUP]3[/SUP], Choi SJ[SUP]2[/SUP], Kang CK[SUP]1[/SUP], Choe PG[SUP]1[/SUP], Kim JY[SUP]4[/SUP], Yun J[SUP]3[/SUP], Lee GW[SUP]3[/SUP], Seong MW[SUP]5[/SUP], Kim NJ[SUP]1,[/SUP][SUP]2[/SUP], Seo JS[SUP]3,[/SUP][SUP]6[/SUP], Oh MD[SUP]1,[/SUP][SUP]7[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Novel coronavirus (SARS-CoV-2) is found to cause a large outbreak started from Wuhan since December 2019 in China and SARS-CoV-2 infections have been reported with epidemiological linkage to China in 25 countries until now. We isolated SARS-CoV-2 from the oropharyngeal sample obtained from the patient with the first laboratory-confirmed SARS-CoV-2 infection in Korea. Cytopathic effects of SARS-CoV-2 in the Vero cell cultures were confluent 3 days after the first blind passage of the sample. Coronavirus was confirmed with spherical particle having a fringe reminiscent of crown on transmission electron microscopy. Phylogenetic analyses of whole genome sequences showed that it clustered with other SARS-CoV-2 reported from Wuhan.
? 2020 The Korean Academy of Medical Sciences.
[h=4]KEYWORDS:[/h] 2019 Novel Coronavirus; Culture; Korea; Microscopy, Electron; Phylogeny
PMID: 32080990 DOI: 10.3346/jkms.2020.35.e84
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Park WB[SUP]#[/SUP][SUP]1,[/SUP][SUP]2[/SUP], Kwon NJ[SUP]#[/SUP][SUP]3[/SUP], Choi SJ[SUP]2[/SUP], Kang CK[SUP]1[/SUP], Choe PG[SUP]1[/SUP], Kim JY[SUP]4[/SUP], Yun J[SUP]3[/SUP], Lee GW[SUP]3[/SUP], Seong MW[SUP]5[/SUP], Kim NJ[SUP]1,[/SUP][SUP]2[/SUP], Seo JS[SUP]3,[/SUP][SUP]6[/SUP], Oh MD[SUP]1,[/SUP][SUP]7[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Novel coronavirus (SARS-CoV-2) is found to cause a large outbreak started from Wuhan since December 2019 in China and SARS-CoV-2 infections have been reported with epidemiological linkage to China in 25 countries until now. We isolated SARS-CoV-2 from the oropharyngeal sample obtained from the patient with the first laboratory-confirmed SARS-CoV-2 infection in Korea. Cytopathic effects of SARS-CoV-2 in the Vero cell cultures were confluent 3 days after the first blind passage of the sample. Coronavirus was confirmed with spherical particle having a fringe reminiscent of crown on transmission electron microscopy. Phylogenetic analyses of whole genome sequences showed that it clustered with other SARS-CoV-2 reported from Wuhan.
? 2020 The Korean Academy of Medical Sciences.
[h=4]KEYWORDS:[/h] 2019 Novel Coronavirus; Culture; Korea; Microscopy, Electron; Phylogeny
PMID: 32080990 DOI: 10.3346/jkms.2020.35.e84
Free full text