tetano
Editor, Senior Moderator
J King Saud Univ Sci
. 2022 Aug;34(6):102179.
doi: 10.1016/j.jksus.2022.102179. Epub 2022 Jun 16.
A study on the role of aedes mosquitoes in arboviruses and SARS-CoV-2 infection: A new challenge
Shabab Nasir[SUP] 1 [/SUP], Imran Ahmed[SUP] 1 [/SUP], Bilal Hussain[SUP] 1 [/SUP], Muhammad U Ijaz[SUP] 2 [/SUP], Faisal Hafeez[SUP] 3 [/SUP], Mohammad Ahmad Wadaan[SUP] 4 [/SUP], Usman Atique[SUP] 5 [/SUP], Shahid Mahboob[SUP] 4 [/SUP]
Affiliations
Abstract
Chikungunya, Zika, Dengue Viruses, and now Novel Coronavirus are global health challenges that cause human diseases ranging from febrile illnesses to death. Most of these viruses are mainly vectored by Aedes mosquitoes worldwide. Molecular detection of arboviruses was made in female Aedes mosquito pools caught from all the seven districts by using a reliable molecular technique, "RT-PCR." From 216 collections of Aedes species, arboviruses were detected in 27, including only Alphavirus genus to determine mosquito abundance and evaluate the potential role of Aedes aegypti and Ae. albopictus mosquitoes in arboviruses and nvel Coronavirus transmission. 5322 mosquitoes were collected using aspirators; 35.31% (n = 2049) were identified as female Aedes using morphological keys, pooled into 216 pools, and tested for arboviruses and coronaviruses by using RT-PCR with the help of specific primers. Novel Coronavirus was not detected in this study. Only the Flavivirus genus was detected in twenty-seven pools giving an infection rate of 62.96% (n = 17) for DENV2, while DENV3 was 37.03% (n = 10). Furthermore, our results indicated no role of mosquitoes in the spread of Covid-19. Results showed a higher infection rate in urban sites than in rural ones. The detection of arboviruses indicates possible human health risk due to active role of these mosquitoes in spreading of arbovirus in the study area.
Keywords: Aedes aegypti; Aedes albopictus; Arboviruses; Dengue; Mosquito; Novel coronavirus; RT-PCR.
. 2022 Aug;34(6):102179.
doi: 10.1016/j.jksus.2022.102179. Epub 2022 Jun 16.
A study on the role of aedes mosquitoes in arboviruses and SARS-CoV-2 infection: A new challenge
Shabab Nasir[SUP] 1 [/SUP], Imran Ahmed[SUP] 1 [/SUP], Bilal Hussain[SUP] 1 [/SUP], Muhammad U Ijaz[SUP] 2 [/SUP], Faisal Hafeez[SUP] 3 [/SUP], Mohammad Ahmad Wadaan[SUP] 4 [/SUP], Usman Atique[SUP] 5 [/SUP], Shahid Mahboob[SUP] 4 [/SUP]
Affiliations
- PMID: 36000083
- PMCID: PMC9388054
- DOI: 10.1016/j.jksus.2022.102179
Abstract
Chikungunya, Zika, Dengue Viruses, and now Novel Coronavirus are global health challenges that cause human diseases ranging from febrile illnesses to death. Most of these viruses are mainly vectored by Aedes mosquitoes worldwide. Molecular detection of arboviruses was made in female Aedes mosquito pools caught from all the seven districts by using a reliable molecular technique, "RT-PCR." From 216 collections of Aedes species, arboviruses were detected in 27, including only Alphavirus genus to determine mosquito abundance and evaluate the potential role of Aedes aegypti and Ae. albopictus mosquitoes in arboviruses and nvel Coronavirus transmission. 5322 mosquitoes were collected using aspirators; 35.31% (n = 2049) were identified as female Aedes using morphological keys, pooled into 216 pools, and tested for arboviruses and coronaviruses by using RT-PCR with the help of specific primers. Novel Coronavirus was not detected in this study. Only the Flavivirus genus was detected in twenty-seven pools giving an infection rate of 62.96% (n = 17) for DENV2, while DENV3 was 37.03% (n = 10). Furthermore, our results indicated no role of mosquitoes in the spread of Covid-19. Results showed a higher infection rate in urban sites than in rural ones. The detection of arboviruses indicates possible human health risk due to active role of these mosquitoes in spreading of arbovirus in the study area.
Keywords: Aedes aegypti; Aedes albopictus; Arboviruses; Dengue; Mosquito; Novel coronavirus; RT-PCR.