tetano
Editor, Senior Moderator
J Infect Public Health
. 2022 Oct 17;15(11):1315-1320.
doi: 10.1016/j.jiph.2022.10.008. Online ahead of print.
Differential expression of carcinoembryonic antigen-related cell adhesion molecule-5 (CEACAM5) and dipeptidyl peptidase-4 (DPP4) with detection of Middle East respiratory syndrome-coronavirus in peripheral blood
Abdulkarim Alhetheel[SUP] 1 [/SUP], Ahmed Albarrag[SUP] 2 [/SUP], Zahid Shakoor[SUP] 2 [/SUP], Ali Somily[SUP] 2 [/SUP], Mazin Barry[SUP] 3 [/SUP], Hifa Altalhi[SUP] 4 [/SUP], Muhammed Bakhrebah[SUP] 5 [/SUP], Majed Nassar[SUP] 5 [/SUP], Mohamed Alfageeh[SUP] 5 [/SUP], Ayed Assiri[SUP] 6 [/SUP], Sarah Alfaraj[SUP] 7 [/SUP], Ziad A Memish[SUP] 8 [/SUP]
Affiliations
Abstract
Background: Middle East respiratory syndrome-coronavirus (MERS-CoV) utilizes CD26 (dipeptidyl peptidase-4) and CD66e or CEACAM5 (carcinoembryonic antigen-related cell adhesion molecule 5) receptors for cell infection. Peripheral blood mononuclear cells (PBMCs) play a critical role in mounting adaptive immune response against the virus. This study was performed to assess the expression of CD26 and CD66e on PBMCs and their susceptibility to MERS-CoV infection.
Methods: Surface expression of CD26 and CD66e receptors on PBMCs from MERS-CoV patients (n = 20) and healthy controls (n = 20) was assessed by flow cytometry and the soluble forms were determined by enzyme-linked immunosorbent assay (ELISA). MERS-CoV UpE and Orf1a genes in PBMCs were detected by using Altona diagnostics reverse transcription polymerase chain reaction (RT-PCR) kit.
Results: Mean fluorescent intensity (MFI) of CD66e was significantly higher on CD4 + lymphocytes (462.4 ± 64.35 vs 325.1 ± 19.69; p < 0.05) and CD8 + lymphocytes (533.8 ± 55.32 vs 392.4 ± 37.73; p < 0.04) from patients with MERS-CoV infection compared to the normal controls. No difference in MFI for CD66e was observed on monocytes (381.8 ± 40.34 vs 266.8 ± 20.6; p = 0.3) between the patients and controls. Soluble form of CD66e among MERS-CoV patients was also higher than the normal controls (mean= 338.7 ± 58.75 vs 160.7 ± 29.49 ng/mL; p < 0.01). Surface expression of CD26 on PBMCs and its soluble form were no different between the groups. MERS-CoV was detected by RT-PCR in 16/20 (80%) patients from whole blood, among them 8 patients were tested in PBMCs, 4/8 (50%) patients were positive.
Conclusion: Increased expression levels of CD66e (CEACAM5) may contribute to increased susceptibility of PBMCs to MERS-CoV infection and disease progression.
Keywords: CD26; CD66e; CEACAM5; DPP4; MERS-CoV; PBMCs; Saudi Arabia.
. 2022 Oct 17;15(11):1315-1320.
doi: 10.1016/j.jiph.2022.10.008. Online ahead of print.
Differential expression of carcinoembryonic antigen-related cell adhesion molecule-5 (CEACAM5) and dipeptidyl peptidase-4 (DPP4) with detection of Middle East respiratory syndrome-coronavirus in peripheral blood
Abdulkarim Alhetheel[SUP] 1 [/SUP], Ahmed Albarrag[SUP] 2 [/SUP], Zahid Shakoor[SUP] 2 [/SUP], Ali Somily[SUP] 2 [/SUP], Mazin Barry[SUP] 3 [/SUP], Hifa Altalhi[SUP] 4 [/SUP], Muhammed Bakhrebah[SUP] 5 [/SUP], Majed Nassar[SUP] 5 [/SUP], Mohamed Alfageeh[SUP] 5 [/SUP], Ayed Assiri[SUP] 6 [/SUP], Sarah Alfaraj[SUP] 7 [/SUP], Ziad A Memish[SUP] 8 [/SUP]
Affiliations
- PMID: 36279687
- DOI: 10.1016/j.jiph.2022.10.008
Abstract
Background: Middle East respiratory syndrome-coronavirus (MERS-CoV) utilizes CD26 (dipeptidyl peptidase-4) and CD66e or CEACAM5 (carcinoembryonic antigen-related cell adhesion molecule 5) receptors for cell infection. Peripheral blood mononuclear cells (PBMCs) play a critical role in mounting adaptive immune response against the virus. This study was performed to assess the expression of CD26 and CD66e on PBMCs and their susceptibility to MERS-CoV infection.
Methods: Surface expression of CD26 and CD66e receptors on PBMCs from MERS-CoV patients (n = 20) and healthy controls (n = 20) was assessed by flow cytometry and the soluble forms were determined by enzyme-linked immunosorbent assay (ELISA). MERS-CoV UpE and Orf1a genes in PBMCs were detected by using Altona diagnostics reverse transcription polymerase chain reaction (RT-PCR) kit.
Results: Mean fluorescent intensity (MFI) of CD66e was significantly higher on CD4 + lymphocytes (462.4 ± 64.35 vs 325.1 ± 19.69; p < 0.05) and CD8 + lymphocytes (533.8 ± 55.32 vs 392.4 ± 37.73; p < 0.04) from patients with MERS-CoV infection compared to the normal controls. No difference in MFI for CD66e was observed on monocytes (381.8 ± 40.34 vs 266.8 ± 20.6; p = 0.3) between the patients and controls. Soluble form of CD66e among MERS-CoV patients was also higher than the normal controls (mean= 338.7 ± 58.75 vs 160.7 ± 29.49 ng/mL; p < 0.01). Surface expression of CD26 on PBMCs and its soluble form were no different between the groups. MERS-CoV was detected by RT-PCR in 16/20 (80%) patients from whole blood, among them 8 patients were tested in PBMCs, 4/8 (50%) patients were positive.
Conclusion: Increased expression levels of CD66e (CEACAM5) may contribute to increased susceptibility of PBMCs to MERS-CoV infection and disease progression.
Keywords: CD26; CD66e; CEACAM5; DPP4; MERS-CoV; PBMCs; Saudi Arabia.