tetano
Editor, Senior Moderator
J Infect. 2020 May 7. pii: S0163-4453(20)30244-9. doi: 10.1016/j.jinf.2020.04.033. [Epub ahead of print]
Dynamic profile for the detection of anti-SARS-CoV-2 antibodies using four immunochromatographic assays.
Demey B[SUP]1[/SUP], Daher N[SUP]2[/SUP], Fran?ois C[SUP]1[/SUP], Lanoix JP[SUP]3[/SUP], Duverlie G[SUP]1[/SUP], Castelain S[SUP]1[/SUP], Brochot E[SUP]4[/SUP].
Author information
Abstract
In order to fight the SARS-CoV-2 pandemic infection, there is a growing need and demand for diagnostic tools that are complementary and different from the RT-PCR currently in use. Multiple serological tests are or will be very soon available but need to be evaluated and validated. We have thus tested 4 immunochromatographic tests for the detection of antibodies to SARS-CoV-2. In addition, we assessed the kinetics of antibody appearance using these assays in 22 patients after they were tested positive by RT-PCR. We observed great heterogeneity in antiboy detection post-symptom onset. The median antibody detection time was between 8 and 10 days according to the manufacturers. All the tests showed a sensitivity of 60 to 80% on day 10 and 100% on day 15. In addition, a single cross-reaction was observed with other human coronavirus infections. Thus, immunochromatographic tests for the detection of anti-SARS-CoV-2 antibodies may have their place for the diagnostic panel of COVID-19.
Copyright ? 2020 Elsevier Ltd. All rights reserved.
KEYWORDS:
Antibody; COVID-19; Lateral flow assay; SARS-CoV-2
PMID:32389784DOI:10.1016/j.jinf.2020.04.033
Dynamic profile for the detection of anti-SARS-CoV-2 antibodies using four immunochromatographic assays.
Demey B[SUP]1[/SUP], Daher N[SUP]2[/SUP], Fran?ois C[SUP]1[/SUP], Lanoix JP[SUP]3[/SUP], Duverlie G[SUP]1[/SUP], Castelain S[SUP]1[/SUP], Brochot E[SUP]4[/SUP].
Author information
Abstract
In order to fight the SARS-CoV-2 pandemic infection, there is a growing need and demand for diagnostic tools that are complementary and different from the RT-PCR currently in use. Multiple serological tests are or will be very soon available but need to be evaluated and validated. We have thus tested 4 immunochromatographic tests for the detection of antibodies to SARS-CoV-2. In addition, we assessed the kinetics of antibody appearance using these assays in 22 patients after they were tested positive by RT-PCR. We observed great heterogeneity in antiboy detection post-symptom onset. The median antibody detection time was between 8 and 10 days according to the manufacturers. All the tests showed a sensitivity of 60 to 80% on day 10 and 100% on day 15. In addition, a single cross-reaction was observed with other human coronavirus infections. Thus, immunochromatographic tests for the detection of anti-SARS-CoV-2 antibodies may have their place for the diagnostic panel of COVID-19.
Copyright ? 2020 Elsevier Ltd. All rights reserved.
KEYWORDS:
Antibody; COVID-19; Lateral flow assay; SARS-CoV-2
PMID:32389784DOI:10.1016/j.jinf.2020.04.033