tetano
Editor, Senior Moderator
J Infect Dis. (2014) doi: 10.1093/infdis/jiu009 First published online: January 7, 2014
Massive Mobilization of Dendritic Cells in H5N1 Influenza Virus Infection of Nonhuman Primates
Adam C. Soloff1,
Stephanie J. Bissel2,
Beth Fallert Junecko3,
Brendan M. Giles1,
Todd A. Reinhart3,
Ted M. Ross1,4 and
Simon M. Barratt-Boyes1,3,5
+ Author Affiliations
1Center for Vaccine Research
2Department of Pathology
3Department of Infectious Disease and Microbiology
4Department of Microbiology and Molecular Genetics
5Department of Immunology, University of Pittsburgh, Pittsburgh, Pennsylvania
Correspondence: Address correspondence to Simon M. Barratt-Boyes, 9046 BST3, University of Pittsburgh, Pittsburgh, Pennsylvania , 15261, USA. Phone: 412-383-7537, Fax: 1-412-624-4440, email: smbb@pitt.edu
Abstract
Highly pathogenic avian influenza (HPAI) virus infection is characterized by a marked inflammatory response but the impact of infection on dendritic cells (DC) is unknown. We show that H5N1 influenza virus infection rapidly and profoundly impacts DC in cynomolgus macaques, increasing the number of blood myeloid and plasmacytoid DC by 16- and 60-fold, respectively. Infection was associated with recruitment, activation and apoptosis of DC in lung-draining lymph nodes and granulocyte and macrophage infiltration with expression of CXCL10 in lungs. This degree of DC mobilization is unprecedented in viral infection and suggests a potential role for DC in HPAI pathogenesis.
http://jid.oxfordjournals.org/content/early/2014/01/07/infdis.jiu009.abstract
Massive Mobilization of Dendritic Cells in H5N1 Influenza Virus Infection of Nonhuman Primates
Adam C. Soloff1,
Stephanie J. Bissel2,
Beth Fallert Junecko3,
Brendan M. Giles1,
Todd A. Reinhart3,
Ted M. Ross1,4 and
Simon M. Barratt-Boyes1,3,5
+ Author Affiliations
1Center for Vaccine Research
2Department of Pathology
3Department of Infectious Disease and Microbiology
4Department of Microbiology and Molecular Genetics
5Department of Immunology, University of Pittsburgh, Pittsburgh, Pennsylvania
Correspondence: Address correspondence to Simon M. Barratt-Boyes, 9046 BST3, University of Pittsburgh, Pittsburgh, Pennsylvania , 15261, USA. Phone: 412-383-7537, Fax: 1-412-624-4440, email: smbb@pitt.edu
Abstract
Highly pathogenic avian influenza (HPAI) virus infection is characterized by a marked inflammatory response but the impact of infection on dendritic cells (DC) is unknown. We show that H5N1 influenza virus infection rapidly and profoundly impacts DC in cynomolgus macaques, increasing the number of blood myeloid and plasmacytoid DC by 16- and 60-fold, respectively. Infection was associated with recruitment, activation and apoptosis of DC in lung-draining lymph nodes and granulocyte and macrophage infiltration with expression of CXCL10 in lungs. This degree of DC mobilization is unprecedented in viral infection and suggests a potential role for DC in HPAI pathogenesis.
http://jid.oxfordjournals.org/content/early/2014/01/07/infdis.jiu009.abstract