Giuseppe
Emeritus
[Source: Journal of Infectious Diseases, full page: (LINK). Abstract, edited.]
Investigation of anti-MERS-Coronavirus antibodies in blood donors and abbatoir workers in Jeddah and Makkah, Kingdom of Saudi Arabia, fall 2012
Asad S. Aburizaiza 1,#, Frank M. Mattes 2,3,#, Esam I. Azhar 2,4, Ahmed M. Hassan 2, Ziad A. Memish 5, Doreen Muth 6, Benjamin Meyer 6, Eric Lattwein 7, Marcel M?ller 6 and Christian Drosten 6,*
Author Affiliations: <SUP>1</SUP>Enviromental Science Department, Faculty of Metrology, Environmental Science & Arid Land Agriculture, King Abdulaziz University, Jeddah, Saudi Arabia; <SUP>2</SUP>Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia; <SUP>3</SUP>German International Cooperation, Eschborn, Germany; <SUP>4</SUP>Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia; <SUP>5</SUP>Ministry of Health, Riyadh, Saudi Arabia; <SUP>6</SUP>Institute of Virology, University of Bonn Medical Centre, Bonn, Germany; <SUP>7</SUP>Euroimmun, L?beck, Germany
*Corresponding author contact information: Professor Christian Drosten, MD, Institute of Virology, University of Bonn Medical Centre, Sigmund Freud Str 25, 53105 Bonn, Germany, Phone: +49-228-287-11055, Fax: +49-228-287-19144, E-mail: drosten@virology-bonn.de
# Authors have equally contributed to the study
<CITE><ABBR>J Infect Dis.</ABBR> (2013) doi: 10.1093/infdis/jit589 - </CITE>First published online: November 11, 2013
Abstract
Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a novel, potentially zoonotic human coronavirus (HCoV). We investigated MERS-CoV antibodies using a staged approach involving immunofluorescence assay (IFA), differential recombinant IFA, and plaque reduction serum neutralization assay. In 130 blood donors sampled during 2012 in Jeddah, and 226 abbatoir workers sampled in October 2012 in Jeddah and Makkah, 8 reactive sera were seen in IFA but were resolved to be specific for established HCoVs by discriminative testing. There is no evidence MERS-CoV circulated widely in the study region in fall 2012, matching an apparent absence of exported disease during the 2012 Hajj.
Received July 19, 2013. Revision received September 10, 2013. Accepted September 26, 2013.
? The Author 2013. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved.
For Permissions, please e-mail: journals.permissions@oup.com.
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Investigation of anti-MERS-Coronavirus antibodies in blood donors and abbatoir workers in Jeddah and Makkah, Kingdom of Saudi Arabia, fall 2012
Asad S. Aburizaiza 1,#, Frank M. Mattes 2,3,#, Esam I. Azhar 2,4, Ahmed M. Hassan 2, Ziad A. Memish 5, Doreen Muth 6, Benjamin Meyer 6, Eric Lattwein 7, Marcel M?ller 6 and Christian Drosten 6,*
Author Affiliations: <SUP>1</SUP>Enviromental Science Department, Faculty of Metrology, Environmental Science & Arid Land Agriculture, King Abdulaziz University, Jeddah, Saudi Arabia; <SUP>2</SUP>Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia; <SUP>3</SUP>German International Cooperation, Eschborn, Germany; <SUP>4</SUP>Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia; <SUP>5</SUP>Ministry of Health, Riyadh, Saudi Arabia; <SUP>6</SUP>Institute of Virology, University of Bonn Medical Centre, Bonn, Germany; <SUP>7</SUP>Euroimmun, L?beck, Germany
*Corresponding author contact information: Professor Christian Drosten, MD, Institute of Virology, University of Bonn Medical Centre, Sigmund Freud Str 25, 53105 Bonn, Germany, Phone: +49-228-287-11055, Fax: +49-228-287-19144, E-mail: drosten@virology-bonn.de
# Authors have equally contributed to the study
<CITE><ABBR>J Infect Dis.</ABBR> (2013) doi: 10.1093/infdis/jit589 - </CITE>First published online: November 11, 2013
Abstract
Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a novel, potentially zoonotic human coronavirus (HCoV). We investigated MERS-CoV antibodies using a staged approach involving immunofluorescence assay (IFA), differential recombinant IFA, and plaque reduction serum neutralization assay. In 130 blood donors sampled during 2012 in Jeddah, and 226 abbatoir workers sampled in October 2012 in Jeddah and Makkah, 8 reactive sera were seen in IFA but were resolved to be specific for established HCoVs by discriminative testing. There is no evidence MERS-CoV circulated widely in the study region in fall 2012, matching an apparent absence of exported disease during the 2012 Hajj.
Received July 19, 2013. Revision received September 10, 2013. Accepted September 26, 2013.
? The Author 2013. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved.
For Permissions, please e-mail: journals.permissions@oup.com.
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