Giuseppe
Emeritus
[Source: The Journal of Infectious Diseases, full text: (LINK). Abstract, edited.]
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Evaluation of the humoral and cellular immune responses elicited by the live attenuated and inactivated influenza vaccines and their roles in heterologous protection in ferrets
Xing Cheng, James Zengel, Amorsolo L. Suguitan Jr, Qi Xu, Weijia Wang, Jim Lin and Hong Jin*
*Correspondence: Hong Jin, Ph.D, MedImmune, 297 North Bernardo Ave, Mountain View, CA 94043, jinh@medimmune.com, Tel: 650 603 2367
Abstract
The humoral and cellular immune responses elicited by the trivalent live attenuated (LAIV) and inactivated (TIV) influenza vaccines were evaluated in the ferret model using newly developed ferret immunological reagents and assays. In contrast to the TIV, which only induced immune responses in primed animals, LAIV induced strong influenza-specific serum antibody and T cell responses in both na?ve and influenza-seropositive animals. The LAIV offered significant protection against a heterologous H1N1 virus challenge infection in the upper respiratory tract. Influenza-specific IgA and IgG antibody secreting cells (ASCs) and influenza-specific CD4<SUP>+</SUP> and CD8<SUP>+</SUP> T cells were detected in the circulation and local paratracheal draining lymph nodes. The frequency of the influenza-specific ASCs in the local lymph nodes appeared to correlate with the degree of protection in the upper respiratory tract. The protection conferred by LAIV could be attributed not only to the antibody response but also to the cell-mediated and local mucosal immune responses, particularly in na?ve ferrets. These findings may explain why the LAIV is immunologically superior and offers immediate protection after a single dose in children.
Received December 10, 2012. Revision received February 12, 2013. Accepted February 13, 2013.
? The Author 2013. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved.
For Permissions, please e-mail: journals.permissions@oup.com.
-Xing Cheng, James Zengel, Amorsolo L. Suguitan Jr, Qi Xu, Weijia Wang, Jim Lin and Hong Jin*
*Correspondence: Hong Jin, Ph.D, MedImmune, 297 North Bernardo Ave, Mountain View, CA 94043, jinh@medimmune.com, Tel: 650 603 2367
Abstract
The humoral and cellular immune responses elicited by the trivalent live attenuated (LAIV) and inactivated (TIV) influenza vaccines were evaluated in the ferret model using newly developed ferret immunological reagents and assays. In contrast to the TIV, which only induced immune responses in primed animals, LAIV induced strong influenza-specific serum antibody and T cell responses in both na?ve and influenza-seropositive animals. The LAIV offered significant protection against a heterologous H1N1 virus challenge infection in the upper respiratory tract. Influenza-specific IgA and IgG antibody secreting cells (ASCs) and influenza-specific CD4<SUP>+</SUP> and CD8<SUP>+</SUP> T cells were detected in the circulation and local paratracheal draining lymph nodes. The frequency of the influenza-specific ASCs in the local lymph nodes appeared to correlate with the degree of protection in the upper respiratory tract. The protection conferred by LAIV could be attributed not only to the antibody response but also to the cell-mediated and local mucosal immune responses, particularly in na?ve ferrets. These findings may explain why the LAIV is immunologically superior and offers immediate protection after a single dose in children.
Received December 10, 2012. Revision received February 12, 2013. Accepted February 13, 2013.
? The Author 2013. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved.
For Permissions, please e-mail: journals.permissions@oup.com.
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