tetano
Editor, Senior Moderator
J Infect Dis
. 2023 Apr 27;jiad089.
doi: 10.1093/infdis/jiad089. Online ahead of print. Ethanol vapor inhalation treatment inhibits lethal respiratory viral infection with Influenza A
Miho Tamai[SUP] 1 [/SUP], Seita Taba[SUP] 2 [/SUP], Takeshi Mise[SUP] 2 [/SUP], Masao Yamashita[SUP] 2 [/SUP], Hiroki Ishikawa[SUP] 1 [/SUP], Tsumoru Shintake[SUP] 2 [/SUP]
Affiliations
Ethanol (EtOH) effectively inactivates enveloped viruses in vitro, including influenza and SARS-CoV-2. Inhaled EtOH vapor may inhibit viral infection in mammalian respiratory tracts, but this has not yet been demonstrated. Here we report that unexpectedly low EtOH concentrations in solution, approximately 20% (v/v), rapidly inactivate influenza A virus (IAV) at mammalian body temperature (37°C) and are not toxic to lung epithelial cells upon apical exposure. Furthermore, brief exposure to 20% (v/v) EtOH decreases production of infectious progeny viruses in IAV-infected cells. Using an EtOH vapor exposure system that is expected to expose murine respiratory tracts to 20% (v/v) EtOH solution by gas-liquid equilibrium at 37°C, we demonstrate that brief EtOH vapor inhalation twice a day protects mice from lethal IAV respiratory infection by reducing viruses in the lungs without harmful side effects. Our data suggest that EtOH vapor inhalation may provide a versatile therapy against various respiratory viral infectious diseases.
Keywords: Ethanol; influenza; inhalation treatment; respiratory infection; vapor; virucidal.
. 2023 Apr 27;jiad089.
doi: 10.1093/infdis/jiad089. Online ahead of print. Ethanol vapor inhalation treatment inhibits lethal respiratory viral infection with Influenza A
Miho Tamai[SUP] 1 [/SUP], Seita Taba[SUP] 2 [/SUP], Takeshi Mise[SUP] 2 [/SUP], Masao Yamashita[SUP] 2 [/SUP], Hiroki Ishikawa[SUP] 1 [/SUP], Tsumoru Shintake[SUP] 2 [/SUP]
Affiliations
- PMID: 37101418
- DOI: 10.1093/infdis/jiad089
Ethanol (EtOH) effectively inactivates enveloped viruses in vitro, including influenza and SARS-CoV-2. Inhaled EtOH vapor may inhibit viral infection in mammalian respiratory tracts, but this has not yet been demonstrated. Here we report that unexpectedly low EtOH concentrations in solution, approximately 20% (v/v), rapidly inactivate influenza A virus (IAV) at mammalian body temperature (37°C) and are not toxic to lung epithelial cells upon apical exposure. Furthermore, brief exposure to 20% (v/v) EtOH decreases production of infectious progeny viruses in IAV-infected cells. Using an EtOH vapor exposure system that is expected to expose murine respiratory tracts to 20% (v/v) EtOH solution by gas-liquid equilibrium at 37°C, we demonstrate that brief EtOH vapor inhalation twice a day protects mice from lethal IAV respiratory infection by reducing viruses in the lungs without harmful side effects. Our data suggest that EtOH vapor inhalation may provide a versatile therapy against various respiratory viral infectious diseases.
Keywords: Ethanol; influenza; inhalation treatment; respiratory infection; vapor; virucidal.