tetano
Editor, Senior Moderator
J Infect Dis
. 2024 Jul 25;230(1):5-14.
doi: 10.1093/infdis/jiae048. Cytotoxic Lymphocyte-Monocyte Complex Reflects the Dynamics of Coronavirus Disease 2019 Systemic Immune Response
Jiajia Lin[SUP] 1 2 [/SUP], Shiyu Bai[SUP] 2 [/SUP], Liheng He[SUP] 1 [/SUP], Ye Yang[SUP] 3 [/SUP], Xiyue Li[SUP] 1 2 [/SUP], Liulin Luo[SUP] 4 [/SUP], Ying Wang[SUP] 1 5 [/SUP], Ying-Ying Chen[SUP] 1 5 [/SUP], Jinhong Qin[SUP] 1 [/SUP], Yi Zhong[SUP] 2 [/SUP]
Affiliations
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection causes a variety of clinical manifestations, many of which originate from altered immune responses, either locally or systemically. Immune cell cross-talk occurs mainly in lymphoid organs. However, systemic cell interaction specific to coronavirus disease 2019 has not been well characterized. Here, by employing single-cell RNA sequencing and imaging flow cytometry analysis, we unraveled, in peripheral blood, a heterogeneous group of cell complexes formed by the adherence of CD14+ monocytes to different cytotoxic lymphocytes, including SARS-CoV-2-specific CD8+ T cells, γδ T cells, and natural killer T cells. These lymphocytes attached to CD14+ monocytes that showed enhanced inflammasome activation and pyroptosis-induced cell death in progression stage; in contrast, in the convalescent phase, CD14+ monocytes with elevated antigen presentation potential were targeted by cytotoxic lymphocytes, thereby restricting the excessive immune activation. Collectively, our study reports previously unrecognized cell-cell interplay in the SARS-CoV-2-specific immune response, providing new insight into the intricacy of dynamic immune cell interaction representing antiviral defense.
Keywords: COVID-19; cytotoxic lymphocytes; immune cell interaction; monocytes; single-cell RNA sequencing.
. 2024 Jul 25;230(1):5-14.
doi: 10.1093/infdis/jiae048. Cytotoxic Lymphocyte-Monocyte Complex Reflects the Dynamics of Coronavirus Disease 2019 Systemic Immune Response
Jiajia Lin[SUP] 1 2 [/SUP], Shiyu Bai[SUP] 2 [/SUP], Liheng He[SUP] 1 [/SUP], Ye Yang[SUP] 3 [/SUP], Xiyue Li[SUP] 1 2 [/SUP], Liulin Luo[SUP] 4 [/SUP], Ying Wang[SUP] 1 5 [/SUP], Ying-Ying Chen[SUP] 1 5 [/SUP], Jinhong Qin[SUP] 1 [/SUP], Yi Zhong[SUP] 2 [/SUP]
Affiliations
- PMID: 39052699
- DOI: 10.1093/infdis/jiae048
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection causes a variety of clinical manifestations, many of which originate from altered immune responses, either locally or systemically. Immune cell cross-talk occurs mainly in lymphoid organs. However, systemic cell interaction specific to coronavirus disease 2019 has not been well characterized. Here, by employing single-cell RNA sequencing and imaging flow cytometry analysis, we unraveled, in peripheral blood, a heterogeneous group of cell complexes formed by the adherence of CD14+ monocytes to different cytotoxic lymphocytes, including SARS-CoV-2-specific CD8+ T cells, γδ T cells, and natural killer T cells. These lymphocytes attached to CD14+ monocytes that showed enhanced inflammasome activation and pyroptosis-induced cell death in progression stage; in contrast, in the convalescent phase, CD14+ monocytes with elevated antigen presentation potential were targeted by cytotoxic lymphocytes, thereby restricting the excessive immune activation. Collectively, our study reports previously unrecognized cell-cell interplay in the SARS-CoV-2-specific immune response, providing new insight into the intricacy of dynamic immune cell interaction representing antiviral defense.
Keywords: COVID-19; cytotoxic lymphocytes; immune cell interaction; monocytes; single-cell RNA sequencing.