tetano
Editor, Senior Moderator
J Infect Dis. 2020 May 8. pii: jiaa243. doi: 10.1093/infdis/jiaa243. [Epub ahead of print]
A Peptide-based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Coronavirus Disease 2019 (COVID-19).
Cai XF[SUP]1[/SUP], Chen J[SUP]1[/SUP], Hu JL[SUP]1[/SUP], Long QX[SUP]1[/SUP], Deng HJ[SUP]1[/SUP], Fan K[SUP]2[/SUP], Liao P[SUP]3[/SUP], Liu BZ[SUP]4[/SUP], Wu GC[SUP]5[/SUP], Chen YK[SUP]6[/SUP], Li ZJ[SUP]3[/SUP], Wang K[SUP]3[/SUP], Zhang XL[SUP]4[/SUP], Tian WG[SUP]4[/SUP], Xiang JL[SUP]5[/SUP], Du HX[SUP]5[/SUP], Wang J[SUP]6[/SUP], Hu Y[SUP]1[/SUP], Tang N[SUP]1[/SUP], Lin Y[SUP]1[/SUP], Ren JH[SUP]1[/SUP], Huang LY[SUP]1[/SUP], Wei J[SUP]1[/SUP], Gan CY[SUP]1[/SUP], Chen YM[SUP]1[/SUP], Gao QZ[SUP]1[/SUP], Chen AM[SUP]1[/SUP], He CL[SUP]1[/SUP], Wang DX[SUP]7[/SUP], Hu P[SUP]1[/SUP], Zhou FC[SUP]8[/SUP], Huang AL[SUP]1[/SUP], Liu P[SUP]8[/SUP], Wang DQ[SUP]9[/SUP].
Author information
Abstract
SARS-CoV-2, a novel ?-coronavirus, cause severe pneumonia and has spread throughout the globe rapidly. The disease associated with SARS-CoV-2 infection is named COVID-19. To date, real-time RT-PCR is the only test able to confirm this infection. However, the accuracy of RT-PCR depends on several factors; variations in these factors might significantly lower the sensitivity of detection. Here, we developed a peptide-based luminescent immunoassay that detected immunoglobulin G (IgG) and IgM. The assay cut-off value was determined by evaluating the sera from healthy and infected patients for pathogens other than SARS-CoV-2. To evaluate assay performance, we detected IgG and IgM in the sera from confirmed patients. The positive rate of IgG and IgM was 71.4% and 57.2%, respectively. Therefore, combining our immunoassay with real-time RT-PCR might enhance the diagnostic accuracy of COVID-19.
? The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.
KEYWORDS:
COVID-19; SARS-CoV-2; Serological Test; chemiluminescence immunoassay
PMID:32382737DOI:10.1093/infdis/jiaa243
A Peptide-based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Coronavirus Disease 2019 (COVID-19).
Cai XF[SUP]1[/SUP], Chen J[SUP]1[/SUP], Hu JL[SUP]1[/SUP], Long QX[SUP]1[/SUP], Deng HJ[SUP]1[/SUP], Fan K[SUP]2[/SUP], Liao P[SUP]3[/SUP], Liu BZ[SUP]4[/SUP], Wu GC[SUP]5[/SUP], Chen YK[SUP]6[/SUP], Li ZJ[SUP]3[/SUP], Wang K[SUP]3[/SUP], Zhang XL[SUP]4[/SUP], Tian WG[SUP]4[/SUP], Xiang JL[SUP]5[/SUP], Du HX[SUP]5[/SUP], Wang J[SUP]6[/SUP], Hu Y[SUP]1[/SUP], Tang N[SUP]1[/SUP], Lin Y[SUP]1[/SUP], Ren JH[SUP]1[/SUP], Huang LY[SUP]1[/SUP], Wei J[SUP]1[/SUP], Gan CY[SUP]1[/SUP], Chen YM[SUP]1[/SUP], Gao QZ[SUP]1[/SUP], Chen AM[SUP]1[/SUP], He CL[SUP]1[/SUP], Wang DX[SUP]7[/SUP], Hu P[SUP]1[/SUP], Zhou FC[SUP]8[/SUP], Huang AL[SUP]1[/SUP], Liu P[SUP]8[/SUP], Wang DQ[SUP]9[/SUP].
Author information
Abstract
SARS-CoV-2, a novel ?-coronavirus, cause severe pneumonia and has spread throughout the globe rapidly. The disease associated with SARS-CoV-2 infection is named COVID-19. To date, real-time RT-PCR is the only test able to confirm this infection. However, the accuracy of RT-PCR depends on several factors; variations in these factors might significantly lower the sensitivity of detection. Here, we developed a peptide-based luminescent immunoassay that detected immunoglobulin G (IgG) and IgM. The assay cut-off value was determined by evaluating the sera from healthy and infected patients for pathogens other than SARS-CoV-2. To evaluate assay performance, we detected IgG and IgM in the sera from confirmed patients. The positive rate of IgG and IgM was 71.4% and 57.2%, respectively. Therefore, combining our immunoassay with real-time RT-PCR might enhance the diagnostic accuracy of COVID-19.
? The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.
KEYWORDS:
COVID-19; SARS-CoV-2; Serological Test; chemiluminescence immunoassay
PMID:32382737DOI:10.1093/infdis/jiaa243