tetano
Editor, Senior Moderator
J Infect Dis
. 2025 Apr 17:jiaf202.
doi: 10.1093/infdis/jiaf202. Online ahead of print. A Novel Antibody Against the Non-RBD Region of Middle East Respiratory Syndrome Coronavirus Spike Protein
So-Young Lee[SUP] 1 [/SUP], Hye-Min Woo[SUP] 1 [/SUP], Hyunbum Jeon[SUP] 2 [/SUP], Na-Young Kim[SUP] 1 [/SUP], Da Sol Kim[SUP] 1 [/SUP], Chan Ki Park[SUP] 1 [/SUP], Hyun-Joo Kim[SUP] 1 [/SUP], Kyung-Chang Kim[SUP] 1 [/SUP], Joo-Yeon Lee[SUP] 3 [/SUP], Kunwoong Park[SUP] 2 [/SUP], Youngki Yoo[SUP] 2 [/SUP], Kiju Choi[SUP] 2 [/SUP], Hansaem Lee[SUP] 1 [/SUP]
Affiliations
The Middle East respiratory syndrome coronavirus (MERS-CoV) was first identified in 2012 and has since spread worldwide. To date, no vaccines or therapeutics against MERS have been approved for clinical use. The spike (S) protein of MERS-CoV facilitates attachment and fusion with target cell membranes. Therefore, inhibiting S protein attachment represents a key therapeutic strategy for treating early MERS-CoV infection. Herein, we present seven human neutralizing antibodies (KNIH-58, -68, -72, -78, -88, -90, and -95) against MERS-CoV. KNIH-58 and -68 bound to the receptor-binding subdomain (RBD) of the spike protein, while the other five monoclonal antibodies (mAbs) did not. KNIH-88, which targets the non-RBD region, exhibited potent neutralizing activities in vitro and in a transgenic mouse model, with similar results for KNIH-58. Structural analysis of KNIH-88 bound to the spike protein revealed novel epitopes in the non-RBD region. These findings may facilitate therapeutic and prophylactic antibody development against MERS-CoV.
Keywords: Middle East respiratory syndrome coronavirus (MERS); monoclonal antibodies (mAb); neutralizing antibody; receptor-binding domain; spike protein.
. 2025 Apr 17:jiaf202.
doi: 10.1093/infdis/jiaf202. Online ahead of print. A Novel Antibody Against the Non-RBD Region of Middle East Respiratory Syndrome Coronavirus Spike Protein
So-Young Lee[SUP] 1 [/SUP], Hye-Min Woo[SUP] 1 [/SUP], Hyunbum Jeon[SUP] 2 [/SUP], Na-Young Kim[SUP] 1 [/SUP], Da Sol Kim[SUP] 1 [/SUP], Chan Ki Park[SUP] 1 [/SUP], Hyun-Joo Kim[SUP] 1 [/SUP], Kyung-Chang Kim[SUP] 1 [/SUP], Joo-Yeon Lee[SUP] 3 [/SUP], Kunwoong Park[SUP] 2 [/SUP], Youngki Yoo[SUP] 2 [/SUP], Kiju Choi[SUP] 2 [/SUP], Hansaem Lee[SUP] 1 [/SUP]
Affiliations
- PMID: 40241666
- DOI: 10.1093/infdis/jiaf202
The Middle East respiratory syndrome coronavirus (MERS-CoV) was first identified in 2012 and has since spread worldwide. To date, no vaccines or therapeutics against MERS have been approved for clinical use. The spike (S) protein of MERS-CoV facilitates attachment and fusion with target cell membranes. Therefore, inhibiting S protein attachment represents a key therapeutic strategy for treating early MERS-CoV infection. Herein, we present seven human neutralizing antibodies (KNIH-58, -68, -72, -78, -88, -90, and -95) against MERS-CoV. KNIH-58 and -68 bound to the receptor-binding subdomain (RBD) of the spike protein, while the other five monoclonal antibodies (mAbs) did not. KNIH-88, which targets the non-RBD region, exhibited potent neutralizing activities in vitro and in a transgenic mouse model, with similar results for KNIH-58. Structural analysis of KNIH-88 bound to the spike protein revealed novel epitopes in the non-RBD region. These findings may facilitate therapeutic and prophylactic antibody development against MERS-CoV.
Keywords: Middle East respiratory syndrome coronavirus (MERS); monoclonal antibodies (mAb); neutralizing antibody; receptor-binding domain; spike protein.