tetano
Editor, Senior Moderator
J Infect Dis
. 2021 Apr 27;jiab222.
doi: 10.1093/infdis/jiab222. Online ahead of print.
A combination of N and S antigens with IgA and IgG measurement strengthens the accuracy of SARS-CoV-2 serodiagnostics
Pinja Jalkanen[SUP] 1 [/SUP], Arja Pasternack[SUP] 2 [/SUP], Sari Maljanen[SUP] 1 [/SUP], Krister Mel?n[SUP] 1 3 [/SUP], Pekka Kolehmainen[SUP] 1 [/SUP], Moona Huttunen[SUP] 1 [/SUP], Rickard Lundberg[SUP] 1 [/SUP], Lav Tripathi[SUP] 1 [/SUP], Hira Khan[SUP] 1 [/SUP], Mikael A Ritvos[SUP] 2 4 5 [/SUP], Rauno Naves[SUP] 2 [/SUP], Anu Haveri[SUP] 3 [/SUP], Pamela ?sterlund[SUP] 3 [/SUP], Suvi Kuivanen[SUP] 6 [/SUP], Anne J J??skel?inen[SUP] 7 [/SUP], Satu Kurkela[SUP] 7 [/SUP], Maija Lappalainen[SUP] 7 [/SUP], Kaisa Rantas?rkk?[SUP] 8 [/SUP], Tytti Vuorinen[SUP] 1 8 [/SUP], Jukka Hyt?nen[SUP] 1 8 [/SUP], Matti Waris[SUP] 1 8 [/SUP], Sisko Tauriainen[SUP] 1 [/SUP], Olli Ritvos[SUP] 2 [/SUP], Laura Kakkola[SUP] 1 [/SUP], Ilkka Julkunen[SUP] 1 8 [/SUP]
Affiliations
Abstract
Background: The primary diagnosis of SARS-CoV-2 infection is based on the detection of virus RNA in nasopharyngeal swab samples. In addition, analysis of humoral immunity against SARS-CoV-2 has an important role in viral diagnostics and seroprevalence estimates.
Methods: We developed and optimized an enzyme immunoassays (EIA) using SARS-CoV-2 nucleoprotein (N), S1 and receptor binding domain (RBD) of the viral spike protein, and N proteins from SARS, MERS, and four low-pathogenic human CoVs. Neutralizing antibody activity was compared with SARS-CoV-2 IgG, IgA and IgM EIA results.
Results: The sensitivity of EIA for detecting immune response in COVID-19 patients (n=101) was 77 % in the acute phase and 100 % in the convalescent phase of SARS-CoV-2 infection when N and RBD were used as antigens in IgG and IgA specific EIAs. SARS-CoV-2 infection significantly increased humoral immune responses against the 229E and NL63 N proteins. S1 and RBD-based EIA results had a strong correlation with microneutralization test (MNT) results.
Conclusions: The data indicate that a combination of SARS-CoV-2 S1 or RBD and N proteins and analysis of sera in IgG and IgA immunoglobulin classes provide an excellent basis for specific and sensitive serological diagnostics of COVID-19.
Keywords: COVID-19; SARS-CoV-2; antibodies; coronavirus proteins; enzyme immunoassay; neutralizing antibodies; respiratory infection; serology.
. 2021 Apr 27;jiab222.
doi: 10.1093/infdis/jiab222. Online ahead of print.
A combination of N and S antigens with IgA and IgG measurement strengthens the accuracy of SARS-CoV-2 serodiagnostics
Pinja Jalkanen[SUP] 1 [/SUP], Arja Pasternack[SUP] 2 [/SUP], Sari Maljanen[SUP] 1 [/SUP], Krister Mel?n[SUP] 1 3 [/SUP], Pekka Kolehmainen[SUP] 1 [/SUP], Moona Huttunen[SUP] 1 [/SUP], Rickard Lundberg[SUP] 1 [/SUP], Lav Tripathi[SUP] 1 [/SUP], Hira Khan[SUP] 1 [/SUP], Mikael A Ritvos[SUP] 2 4 5 [/SUP], Rauno Naves[SUP] 2 [/SUP], Anu Haveri[SUP] 3 [/SUP], Pamela ?sterlund[SUP] 3 [/SUP], Suvi Kuivanen[SUP] 6 [/SUP], Anne J J??skel?inen[SUP] 7 [/SUP], Satu Kurkela[SUP] 7 [/SUP], Maija Lappalainen[SUP] 7 [/SUP], Kaisa Rantas?rkk?[SUP] 8 [/SUP], Tytti Vuorinen[SUP] 1 8 [/SUP], Jukka Hyt?nen[SUP] 1 8 [/SUP], Matti Waris[SUP] 1 8 [/SUP], Sisko Tauriainen[SUP] 1 [/SUP], Olli Ritvos[SUP] 2 [/SUP], Laura Kakkola[SUP] 1 [/SUP], Ilkka Julkunen[SUP] 1 8 [/SUP]
Affiliations
- PMID: 33905505
- DOI: 10.1093/infdis/jiab222
Abstract
Background: The primary diagnosis of SARS-CoV-2 infection is based on the detection of virus RNA in nasopharyngeal swab samples. In addition, analysis of humoral immunity against SARS-CoV-2 has an important role in viral diagnostics and seroprevalence estimates.
Methods: We developed and optimized an enzyme immunoassays (EIA) using SARS-CoV-2 nucleoprotein (N), S1 and receptor binding domain (RBD) of the viral spike protein, and N proteins from SARS, MERS, and four low-pathogenic human CoVs. Neutralizing antibody activity was compared with SARS-CoV-2 IgG, IgA and IgM EIA results.
Results: The sensitivity of EIA for detecting immune response in COVID-19 patients (n=101) was 77 % in the acute phase and 100 % in the convalescent phase of SARS-CoV-2 infection when N and RBD were used as antigens in IgG and IgA specific EIAs. SARS-CoV-2 infection significantly increased humoral immune responses against the 229E and NL63 N proteins. S1 and RBD-based EIA results had a strong correlation with microneutralization test (MNT) results.
Conclusions: The data indicate that a combination of SARS-CoV-2 S1 or RBD and N proteins and analysis of sera in IgG and IgA immunoglobulin classes provide an excellent basis for specific and sensitive serological diagnostics of COVID-19.
Keywords: COVID-19; SARS-CoV-2; antibodies; coronavirus proteins; enzyme immunoassay; neutralizing antibodies; respiratory infection; serology.