tetano
Editor, Senior Moderator
J Hazard Mater
. 2024 May 17:473:134635.
doi: 10.1016/j.jhazmat.2024.134635. Online ahead of print. Wastewater surveillance together with metaviromic data revealed the unusual resurgence of infectious diseases after the first wave of the COVID-19 outbreak
Ziqiang Zhang[SUP] 1 [/SUP], Fenglan He[SUP] 2 [/SUP], Liu Yi[SUP] 2 [/SUP], Zhiqiang Deng[SUP] 2 [/SUP], Rui Wang[SUP] 3 [/SUP], Lixin Shen[SUP] 4 [/SUP], Songzhe Fu[SUP] 5 [/SUP]
Affiliations
How to address public health priorities after COVID-19 is becoming a critical task. To this end, we conducted wastewater surveillance for six leading pathogens, namely, SARS-CoV-2, norovirus, rotavirus, influenza A virus (IAV), enteroviruses and respiratory syncytial virus (RSV), in Nanchang city from January to April 2023. Metaviromic sequencing was conducted at the 1st, 4th, 7th, 9th, 12th and 14th weeks to reveal the dynamics of viral pathogens that were not covered by qPCR. Amplicon sequencing of the conserved region of norovirus GI and GII and the rotavirus and region encoding nonstructural protein of RSV was also conducted weekly. The results showed that after a rapid decrease in SARS-CoV-2 sewage concentrations occurred in January 2023, surges of norovirus, rotavirus, IAV and RSV started at the 6th, 7th, 8th and 11th weeks, respectively. The dynamics of the sewage concentrations of norovirus, rotavirus, IAV and RSV were consistent with the off-season resurgence of the above infectious diseases. Notably, peak sewage concentrations of norovirus GI, GII, rotavirus, IAV and RSV were found at the 6th, 3rd, 7th, 7th and 8th weeks, respectively. Astroviruses also resurge after the 7th week, as revealed by metaviromic data, suggesting that wastewater surveillance together with metaviromic data provides an essential early warning tool for revealing patterns of infectious disease resurgence.
Keywords: Amplicon sequencing; Metaviromic data; Norovirus; Off-season resurgence; SARS-CoV-2; Wastewater surveillance.
. 2024 May 17:473:134635.
doi: 10.1016/j.jhazmat.2024.134635. Online ahead of print. Wastewater surveillance together with metaviromic data revealed the unusual resurgence of infectious diseases after the first wave of the COVID-19 outbreak
Ziqiang Zhang[SUP] 1 [/SUP], Fenglan He[SUP] 2 [/SUP], Liu Yi[SUP] 2 [/SUP], Zhiqiang Deng[SUP] 2 [/SUP], Rui Wang[SUP] 3 [/SUP], Lixin Shen[SUP] 4 [/SUP], Songzhe Fu[SUP] 5 [/SUP]
Affiliations
- PMID: 38772110
- DOI: 10.1016/j.jhazmat.2024.134635
How to address public health priorities after COVID-19 is becoming a critical task. To this end, we conducted wastewater surveillance for six leading pathogens, namely, SARS-CoV-2, norovirus, rotavirus, influenza A virus (IAV), enteroviruses and respiratory syncytial virus (RSV), in Nanchang city from January to April 2023. Metaviromic sequencing was conducted at the 1st, 4th, 7th, 9th, 12th and 14th weeks to reveal the dynamics of viral pathogens that were not covered by qPCR. Amplicon sequencing of the conserved region of norovirus GI and GII and the rotavirus and region encoding nonstructural protein of RSV was also conducted weekly. The results showed that after a rapid decrease in SARS-CoV-2 sewage concentrations occurred in January 2023, surges of norovirus, rotavirus, IAV and RSV started at the 6th, 7th, 8th and 11th weeks, respectively. The dynamics of the sewage concentrations of norovirus, rotavirus, IAV and RSV were consistent with the off-season resurgence of the above infectious diseases. Notably, peak sewage concentrations of norovirus GI, GII, rotavirus, IAV and RSV were found at the 6th, 3rd, 7th, 7th and 8th weeks, respectively. Astroviruses also resurge after the 7th week, as revealed by metaviromic data, suggesting that wastewater surveillance together with metaviromic data provides an essential early warning tool for revealing patterns of infectious disease resurgence.
Keywords: Amplicon sequencing; Metaviromic data; Norovirus; Off-season resurgence; SARS-CoV-2; Wastewater surveillance.