tetano
Editor, Senior Moderator
J Hazard Mater
. 2024 Dec 15:485:136887.
doi: 10.1016/j.jhazmat.2024.136887. Online ahead of print. The influence of PM2.5 exposure on SARS-CoV-2 infection via modulating the expression of angiotensin converting enzyme II
Meng-Wei Lin[SUP] 1 [/SUP], Cheng-Han Lin[SUP] 2 [/SUP], Jia-Rong Chang[SUP] 3 [/SUP], Hua-Hsin Chiang[SUP] 4 [/SUP], Ting-Hsuan Wu[SUP] 5 [/SUP], Chih-Sheng Lin[SUP] 6 [/SUP]
Affiliations
Particulate matter 2.5 (PM2.5) pollution and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic are the greatest environmental health issues worldwide. Several statistics revealed the significant positive correlation between the morbidity of coronavirus disease-19 (COVID-19) and the levels of air pollution. Nevertheless, there is no direct experimental evidence to indicate the effect of PM2.5 exposure on SARS-CoV-2 infection. The objective of this study was to evaluate whether the infection of SARS-CoV-2 affected by PM2.5 through angiotensin-converting enzyme II (ACE2) expression enhances and investigate the function of ACE2 in lung injury induced by PM2.5. An animal model of PM2.5-induced lung injury was established using wild-type (WT, C57BL/6), human ACE2 transgenic (K18-hACE2 TG), and murine ACE2 gene knockout (mACE2 KO) mice. The results indicate that PM2.5 exposure facilitates SARS-CoV-2 infection through inducing ACE2 expression in vitro (10 μg/mL) and in vivo (6.25 mg/kg/day in 50 μL saline). The levels of ACE, inflammatory cytokines, and mitogen-activated protein kinase (MAPK) proteins in WT, K18-hACE TG and mACE2 KO mice were significantly increased after PM2.5 instillation. The severest PM2.5-induced lung damage was observed in mACE2 KO mice. In summary, ACE2 plays a double-edged sword role in lung injury, PM2.5 exposure contributed to SARS-CoV-2 infection through inducing ACE2 expression, but ACE2 also protected pulmonary inflammation from PM2.5 challenge.
Keywords: Air pollution; Angiotensin converting enzyme II; Inflammation; Particulate matter 2.5; Severe acute respiratory syndrome coronavirus 2.
. 2024 Dec 15:485:136887.
doi: 10.1016/j.jhazmat.2024.136887. Online ahead of print. The influence of PM2.5 exposure on SARS-CoV-2 infection via modulating the expression of angiotensin converting enzyme II
Meng-Wei Lin[SUP] 1 [/SUP], Cheng-Han Lin[SUP] 2 [/SUP], Jia-Rong Chang[SUP] 3 [/SUP], Hua-Hsin Chiang[SUP] 4 [/SUP], Ting-Hsuan Wu[SUP] 5 [/SUP], Chih-Sheng Lin[SUP] 6 [/SUP]
Affiliations
- PMID: 39700942
- DOI: 10.1016/j.jhazmat.2024.136887
Particulate matter 2.5 (PM2.5) pollution and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic are the greatest environmental health issues worldwide. Several statistics revealed the significant positive correlation between the morbidity of coronavirus disease-19 (COVID-19) and the levels of air pollution. Nevertheless, there is no direct experimental evidence to indicate the effect of PM2.5 exposure on SARS-CoV-2 infection. The objective of this study was to evaluate whether the infection of SARS-CoV-2 affected by PM2.5 through angiotensin-converting enzyme II (ACE2) expression enhances and investigate the function of ACE2 in lung injury induced by PM2.5. An animal model of PM2.5-induced lung injury was established using wild-type (WT, C57BL/6), human ACE2 transgenic (K18-hACE2 TG), and murine ACE2 gene knockout (mACE2 KO) mice. The results indicate that PM2.5 exposure facilitates SARS-CoV-2 infection through inducing ACE2 expression in vitro (10 μg/mL) and in vivo (6.25 mg/kg/day in 50 μL saline). The levels of ACE, inflammatory cytokines, and mitogen-activated protein kinase (MAPK) proteins in WT, K18-hACE TG and mACE2 KO mice were significantly increased after PM2.5 instillation. The severest PM2.5-induced lung damage was observed in mACE2 KO mice. In summary, ACE2 plays a double-edged sword role in lung injury, PM2.5 exposure contributed to SARS-CoV-2 infection through inducing ACE2 expression, but ACE2 also protected pulmonary inflammation from PM2.5 challenge.
Keywords: Air pollution; Angiotensin converting enzyme II; Inflammation; Particulate matter 2.5; Severe acute respiratory syndrome coronavirus 2.