tetano
Editor, Senior Moderator
J Hazard Mater
. 2023 Dec 14:465:133249.
doi: 10.1016/j.jhazmat.2023.133249. Online ahead of print. Enhanced enrichment of collected airborne coronavirus and influenza virus samples via a ConA-coated microfluidic chip for PCR detection
Amin Piri[SUP] 1 [/SUP], Kyung-A Hyun[SUP] 2 [/SUP], Hyo-Il Jung[SUP] 3 [/SUP], Kang Sik Nam[SUP] 1 [/SUP], Jungho Hwang[SUP] 4 [/SUP]
Affiliations
The severe acute respiratory syndrome (SARS-CoV-2) outbreak triggered global concern and emphasized the importance of virus monitoring. During a seasonal influenza A outbreak, relatively low concentrations of 10[SUP]3[/SUP]-10[SUP]4[/SUP] viral genome copies are available per 1 m[SUP]3[/SUP] of air, which makes detection and monitoring very challenging because the limit of detection of most polymerase chain reaction (PCR) devices is approximately 10[SUP]3[/SUP] viral genome copies/mL. In response to the urgent need for the rapid detection of airborne coronaviruses and influenza viruses, an electrostatic aerosol-to-hydrosol (ATH) sampler was combined with a concanavalin A (ConA)-coated high-throughput microfluidic chip. The samples were then used for PCR detection. The results revealed that the enrichment capacity of the ATH sampler was 30,000-fold for both HCoV-229E and H1N1 influenza virus, whereas the enrichment capacities provided by the ConA-coated microfluidic chip were 8-fold and 16-fold for HCoV-229E and H1N1 virus, respectively. Thus, the total enrichment capacities of our combined ATH sampler and ConA-coated microfluidic chip were 2.4 × 10[SUP]5[/SUP]-fold and 4.8 × 10[SUP]5[/SUP]-fold for HCoV-229E and H1N1 virus, respectively. This methodology significantly improves PCR detection by providing a higher concentration of viable samples.
Keywords: Coronavirus; Electrostatic sampler; Enrichment; H1N1 influenza virus; Microfluidic chip.
. 2023 Dec 14:465:133249.
doi: 10.1016/j.jhazmat.2023.133249. Online ahead of print. Enhanced enrichment of collected airborne coronavirus and influenza virus samples via a ConA-coated microfluidic chip for PCR detection
Amin Piri[SUP] 1 [/SUP], Kyung-A Hyun[SUP] 2 [/SUP], Hyo-Il Jung[SUP] 3 [/SUP], Kang Sik Nam[SUP] 1 [/SUP], Jungho Hwang[SUP] 4 [/SUP]
Affiliations
- PMID: 38154189
- DOI: 10.1016/j.jhazmat.2023.133249
The severe acute respiratory syndrome (SARS-CoV-2) outbreak triggered global concern and emphasized the importance of virus monitoring. During a seasonal influenza A outbreak, relatively low concentrations of 10[SUP]3[/SUP]-10[SUP]4[/SUP] viral genome copies are available per 1 m[SUP]3[/SUP] of air, which makes detection and monitoring very challenging because the limit of detection of most polymerase chain reaction (PCR) devices is approximately 10[SUP]3[/SUP] viral genome copies/mL. In response to the urgent need for the rapid detection of airborne coronaviruses and influenza viruses, an electrostatic aerosol-to-hydrosol (ATH) sampler was combined with a concanavalin A (ConA)-coated high-throughput microfluidic chip. The samples were then used for PCR detection. The results revealed that the enrichment capacity of the ATH sampler was 30,000-fold for both HCoV-229E and H1N1 influenza virus, whereas the enrichment capacities provided by the ConA-coated microfluidic chip were 8-fold and 16-fold for HCoV-229E and H1N1 virus, respectively. Thus, the total enrichment capacities of our combined ATH sampler and ConA-coated microfluidic chip were 2.4 × 10[SUP]5[/SUP]-fold and 4.8 × 10[SUP]5[/SUP]-fold for HCoV-229E and H1N1 virus, respectively. This methodology significantly improves PCR detection by providing a higher concentration of viable samples.
Keywords: Coronavirus; Electrostatic sampler; Enrichment; H1N1 influenza virus; Microfluidic chip.