tetano
Editor, Senior Moderator
J Gen Virol
. 2020 Sep 9.
doi: 10.1099/jgv.0.001491. Online ahead of print.
Predicting the recombination potential of severe acute respiratory syndrome coronavirus 2 and Middle East respiratory syndrome coronavirus
Arinjay Banerjee[SUP] 1 2 [/SUP], Andrew C Doxey[SUP] 3 4 [/SUP], Benjamin J-M Tremblay[SUP] 3 [/SUP], Michael J Mansfield[SUP] 5 [/SUP], Sonu Subudhi[SUP] 6 [/SUP], Jeremy A Hirota[SUP] 4 1 [/SUP], Matthew S Miller[SUP] 7 1 [/SUP], Andrew G McArthur[SUP] 7 1 [/SUP], Samira Mubareka[SUP] 8 9 [/SUP], Karen Mossman[SUP] 2 1 [/SUP]
Affiliations
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) recently emerged to cause widespread infections in humans. SARS-CoV-2 infections have been reported in the Kingdom of Saudi Arabia, where Middle East respiratory syndrome coronavirus (MERS-CoV) causes seasonal outbreaks with a case fatality rate of ~37 %. Here we show that there exists a theoretical possibility of future recombination events between SARS-CoV-2 and MERS-CoV RNA. Through computational analyses, we have identified homologous genomic regions within the ORF1ab and S genes that could facilitate recombination, and have analysed co-expression patterns of the cellular receptors for SARS-CoV-2 and MERS-CoV, ACE2 and DPP4, respectively, to identify human anatomical sites that could facilitate co-infection. Furthermore, we have investigated the likely susceptibility of various animal species to MERS-CoV and SARS-CoV-2 infection by comparing known virus spike protein-receptor interacting residues. In conclusion, we suggest that a recombination between SARS-CoV-2 and MERS-CoV RNA is possible and urge public health laboratories in high-risk areas to develop diagnostic capability for the detection of recombined coronaviruses in patient samples.
Keywords: MERS-CoV; SARS-CoV-2; coronavirus; emergence; predictions; recombination.
. 2020 Sep 9.
doi: 10.1099/jgv.0.001491. Online ahead of print.
Predicting the recombination potential of severe acute respiratory syndrome coronavirus 2 and Middle East respiratory syndrome coronavirus
Arinjay Banerjee[SUP] 1 2 [/SUP], Andrew C Doxey[SUP] 3 4 [/SUP], Benjamin J-M Tremblay[SUP] 3 [/SUP], Michael J Mansfield[SUP] 5 [/SUP], Sonu Subudhi[SUP] 6 [/SUP], Jeremy A Hirota[SUP] 4 1 [/SUP], Matthew S Miller[SUP] 7 1 [/SUP], Andrew G McArthur[SUP] 7 1 [/SUP], Samira Mubareka[SUP] 8 9 [/SUP], Karen Mossman[SUP] 2 1 [/SUP]
Affiliations
- PMID: 32902372
- DOI: 10.1099/jgv.0.001491
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) recently emerged to cause widespread infections in humans. SARS-CoV-2 infections have been reported in the Kingdom of Saudi Arabia, where Middle East respiratory syndrome coronavirus (MERS-CoV) causes seasonal outbreaks with a case fatality rate of ~37 %. Here we show that there exists a theoretical possibility of future recombination events between SARS-CoV-2 and MERS-CoV RNA. Through computational analyses, we have identified homologous genomic regions within the ORF1ab and S genes that could facilitate recombination, and have analysed co-expression patterns of the cellular receptors for SARS-CoV-2 and MERS-CoV, ACE2 and DPP4, respectively, to identify human anatomical sites that could facilitate co-infection. Furthermore, we have investigated the likely susceptibility of various animal species to MERS-CoV and SARS-CoV-2 infection by comparing known virus spike protein-receptor interacting residues. In conclusion, we suggest that a recombination between SARS-CoV-2 and MERS-CoV RNA is possible and urge public health laboratories in high-risk areas to develop diagnostic capability for the detection of recombined coronaviruses in patient samples.
Keywords: MERS-CoV; SARS-CoV-2; coronavirus; emergence; predictions; recombination.