Giuseppe
Emeritus
J Gen Virol. 2009 Dec 16. [Epub ahead of print]
Phylogeny and genotyping of recent avian low pathogenic H5 subtype influenza viruses from French ducks.
Briand FX, Le Gall-Recul? G, Guillou-Cloarec C, Ogor K, Jestin V. - AFSSA, French Reference Laboratory for Avian Influenza and Newcastle Disease, Ploufragan, France.
H5 low pathogenic avian influenza virus (LPAIV) has the potential to become highly pathogenic and to cause serious problems in animal and public health. AIV surveillance and characterization in both wild and domestic species is therefore necessary. In order to acquire molecular information and to identify possible reassortments in French viruses, we analyzed the entire genome of 5 H5N3, 3 H5N2, and 2 H5N1 LPAIV, isolated in France between 2002 and 2008 mostly from captive ducks (free range commercial poultry or decoy ducks). Some genome sequences showed atypical characteristics such as: the insertion of 1 amino acid (aa) in the PB1 protein of one H5N3, a highly truncated PB1-F2 protein (11 aa in length instead of 90 aa) in one H5N2, and an insertion of 8 aa in the NS1 protein of H5N1. These two last molecular characteristics have never been described. Phylogenetic analysis demonstrated that all genes of French LPAIV clustered within Eurasian avian influenzavirus lineage and fell into at least 2 phylogenetic subgroups, except the closely related matrix protein genes. In addition, the French H5 LPAIV were segregated into 8 genotypes, suggesting that many reassortment events occurred in H5 LPAIV in Europe. However, it is not known whether the reassortment events occurred in wild waterfowl and/or in these captive birds in direct or indirect contact with wild birds.
PMID: 20016038 [PubMed - as supplied by publisher]
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Phylogeny and genotyping of recent avian low pathogenic H5 subtype influenza viruses from French ducks.
Briand FX, Le Gall-Recul? G, Guillou-Cloarec C, Ogor K, Jestin V. - AFSSA, French Reference Laboratory for Avian Influenza and Newcastle Disease, Ploufragan, France.
H5 low pathogenic avian influenza virus (LPAIV) has the potential to become highly pathogenic and to cause serious problems in animal and public health. AIV surveillance and characterization in both wild and domestic species is therefore necessary. In order to acquire molecular information and to identify possible reassortments in French viruses, we analyzed the entire genome of 5 H5N3, 3 H5N2, and 2 H5N1 LPAIV, isolated in France between 2002 and 2008 mostly from captive ducks (free range commercial poultry or decoy ducks). Some genome sequences showed atypical characteristics such as: the insertion of 1 amino acid (aa) in the PB1 protein of one H5N3, a highly truncated PB1-F2 protein (11 aa in length instead of 90 aa) in one H5N2, and an insertion of 8 aa in the NS1 protein of H5N1. These two last molecular characteristics have never been described. Phylogenetic analysis demonstrated that all genes of French LPAIV clustered within Eurasian avian influenzavirus lineage and fell into at least 2 phylogenetic subgroups, except the closely related matrix protein genes. In addition, the French H5 LPAIV were segregated into 8 genotypes, suggesting that many reassortment events occurred in H5 LPAIV in Europe. However, it is not known whether the reassortment events occurred in wild waterfowl and/or in these captive birds in direct or indirect contact with wild birds.
PMID: 20016038 [PubMed - as supplied by publisher]
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