Giuseppe
Emeritus
[Source: US National Library of Medicine, (LINK). Edited.]
J Gen Virol. 2010 Apr 21. [Epub ahead of print]
Inhibition of Host Innate Immune Responses and Pathogenicity of Recombinant Newcastle Disease Viruses Expressing NS1 Genes of Influenza A Viruses.
Kim SH, Samal S. - University of Maryland.
NS1 protein has been associated with the virulence of influenza A viruses. To evaluate the role of NS1 protein in pathogenicity of pandemic H5N1 avian influenza and H1N1 2009 influenza viruses, recombinant Newcastle disease viruses (rNDVs) expressing NS1 proteins were generated. Expression of the NS1 proteins resulted in inhibition of host innate immune responses (IFN-beta and PKR production). In addition, the NS1 proteins were dominantly localized in the nucleus of virus-infected cells. Consequently, expression of the NS1 protein contributed to an increase in pathogenicity of rNDV in chickens. In particular, mutational analysis of H5N1 NS1 protein indicates that both RNA-binding and effector domains synergistically affect viral pathogenicity. Our study also demonstrated that expression of H1N1/09 NS1 resulted in enhanced replication of rNDV in human cells, indicating that function of the NS1 proteins can be host species specific.
PMID: 20410312 [PubMed - as supplied by publisher]
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J Gen Virol. 2010 Apr 21. [Epub ahead of print]
Inhibition of Host Innate Immune Responses and Pathogenicity of Recombinant Newcastle Disease Viruses Expressing NS1 Genes of Influenza A Viruses.
Kim SH, Samal S. - University of Maryland.
NS1 protein has been associated with the virulence of influenza A viruses. To evaluate the role of NS1 protein in pathogenicity of pandemic H5N1 avian influenza and H1N1 2009 influenza viruses, recombinant Newcastle disease viruses (rNDVs) expressing NS1 proteins were generated. Expression of the NS1 proteins resulted in inhibition of host innate immune responses (IFN-beta and PKR production). In addition, the NS1 proteins were dominantly localized in the nucleus of virus-infected cells. Consequently, expression of the NS1 protein contributed to an increase in pathogenicity of rNDV in chickens. In particular, mutational analysis of H5N1 NS1 protein indicates that both RNA-binding and effector domains synergistically affect viral pathogenicity. Our study also demonstrated that expression of H1N1/09 NS1 resulted in enhanced replication of rNDV in human cells, indicating that function of the NS1 proteins can be host species specific.
PMID: 20410312 [PubMed - as supplied by publisher]
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