tetano
Editor, Senior Moderator
J Clin Virol
. 2020 Jul 7;129:104543.
doi: 10.1016/j.jcv.2020.104543. Online ahead of print.
Analysis of Covid-19 and non-Covid-19 viruses, including influenza viruses, to determine the influence of intensive preventive measures in Japan
Yosuke Hirotsu[SUP] 1 [/SUP], Makoto Maejima[SUP] 2 [/SUP], Masahiro Shibusawa[SUP] 2 [/SUP], Kenji Amemiya[SUP] 3 [/SUP], Yuki Nagakubo[SUP] 4 [/SUP], Kazuhiro Hosaka[SUP] 2 [/SUP], Hitomi Sueki[SUP] 2 [/SUP], Hitoshi Mochizuki[SUP] 5 [/SUP], Toshiharu Tsutsui[SUP] 6 [/SUP], Yumiko Kakizaki[SUP] 6 [/SUP], Yoshihiro Miyashita[SUP] 6 [/SUP], Masao Omata[SUP] 7 [/SUP]
Affiliations
Abstract
Background: Severe acute respiratory coronavirus 2 (SARS-CoV-2) has spread and caused death worldwide. Preventive measures and infection control are underway, and some areas show signs of convergence. Other viruses in addition to SARS-CoV-2 cause cold-like symptoms and spread in the winter. However, the extent to which SARS-CoV-2, influenza viruses and other causative viruses have prevailed since implementing preventive measures is unclear.
Objectives: We aim to investigate the incidence of causative viruses and pathogens in patients.
Study design: We collected 191 nasopharyngeal swabs from patients with cold-like symptoms in Japan. All samples were subjected to multiplex PCR with the FilmArray Respiratory Panel and reverse transcription PCR (RT-PCR) to detect SARS-CoV-2.
Results: FilmArray Respiratory Panel analysis detected at least one virus in 32 of 191 patients with cold-like symptoms (21 %). Of these, we frequently identified human rhinoviruses/enteroviruses (5.8 %, n=11), human metapneumovirus (3.7 %, n=7), coronavirus 229E (2.1 %, n=4) and coronavirus OC43 (1.6 %, n=3); while no influenza viruses were detected. RT-PCR analysis detected SARS-CoV-2 (4.2 %, n=8) in patients who were not infected with the aforementioned respiratory viruses.
Conclusions: Co-infection with SARS-CoV-2 and other viruses was not observed. Causative viruses remain prevalent after implementing preventive measures. SARS-CoV-2 differs from influenza viruses in its infectivity.
Keywords: Covid-19; FilmArray; Infection; Influenza; PCR; SARS-CoV-2.
. 2020 Jul 7;129:104543.
doi: 10.1016/j.jcv.2020.104543. Online ahead of print.
Analysis of Covid-19 and non-Covid-19 viruses, including influenza viruses, to determine the influence of intensive preventive measures in Japan
Yosuke Hirotsu[SUP] 1 [/SUP], Makoto Maejima[SUP] 2 [/SUP], Masahiro Shibusawa[SUP] 2 [/SUP], Kenji Amemiya[SUP] 3 [/SUP], Yuki Nagakubo[SUP] 4 [/SUP], Kazuhiro Hosaka[SUP] 2 [/SUP], Hitomi Sueki[SUP] 2 [/SUP], Hitoshi Mochizuki[SUP] 5 [/SUP], Toshiharu Tsutsui[SUP] 6 [/SUP], Yumiko Kakizaki[SUP] 6 [/SUP], Yoshihiro Miyashita[SUP] 6 [/SUP], Masao Omata[SUP] 7 [/SUP]
Affiliations
- PMID: 32663787
- DOI: 10.1016/j.jcv.2020.104543
Abstract
Background: Severe acute respiratory coronavirus 2 (SARS-CoV-2) has spread and caused death worldwide. Preventive measures and infection control are underway, and some areas show signs of convergence. Other viruses in addition to SARS-CoV-2 cause cold-like symptoms and spread in the winter. However, the extent to which SARS-CoV-2, influenza viruses and other causative viruses have prevailed since implementing preventive measures is unclear.
Objectives: We aim to investigate the incidence of causative viruses and pathogens in patients.
Study design: We collected 191 nasopharyngeal swabs from patients with cold-like symptoms in Japan. All samples were subjected to multiplex PCR with the FilmArray Respiratory Panel and reverse transcription PCR (RT-PCR) to detect SARS-CoV-2.
Results: FilmArray Respiratory Panel analysis detected at least one virus in 32 of 191 patients with cold-like symptoms (21 %). Of these, we frequently identified human rhinoviruses/enteroviruses (5.8 %, n=11), human metapneumovirus (3.7 %, n=7), coronavirus 229E (2.1 %, n=4) and coronavirus OC43 (1.6 %, n=3); while no influenza viruses were detected. RT-PCR analysis detected SARS-CoV-2 (4.2 %, n=8) in patients who were not infected with the aforementioned respiratory viruses.
Conclusions: Co-infection with SARS-CoV-2 and other viruses was not observed. Causative viruses remain prevalent after implementing preventive measures. SARS-CoV-2 differs from influenza viruses in its infectivity.
Keywords: Covid-19; FilmArray; Infection; Influenza; PCR; SARS-CoV-2.