tetano
Editor, Senior Moderator
J Clin Microbiol
. 2020 Dec 9;JCM.02676-20.
doi: 10.1128/JCM.02676-20. Online ahead of print.
Variable sensitivity in molecular detection of SARS-CoV-2 in European Expert Laboratories: External Quality Assessment, June - July 2020
Carlo Fischer[SUP] 1 [/SUP], Ramona M?gling[SUP] 2 [/SUP], Angeliki Melidou[SUP] 3 [/SUP], Arne K?hne[SUP] 1 [/SUP], Edmilson F Oliveira-Filho[SUP] 1 [/SUP], Thorsten Wolff[SUP] 4 [/SUP], Janine Reiche[SUP] 4 [/SUP], Eeva Broberg[SUP] 3 [/SUP], Adam Meijer[SUP] 2 [/SUP], Katrin Leitmeyer[SUP] 3 [/SUP], Jan Felix Drexler[SUP] 5 [/SUP], Chantal B E M Reusken[SUP] 6 [/SUP]
Affiliations
Abstract
Objective: During the ongoing coronavirus disease 2019 (COVID-19) outbreak, robust detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a key element for clinical management and to interrupt transmission chains. We organized an external quality assessment (EQA) of molecular detection of SARS-CoV-2 for European expert laboratories.Study design: An EQA panel composed of 12 samples, containing either SARS-CoV-2 at different concentrations to evaluate sensitivity or other respiratory viruses to evaluate specificity of SARS-CoV-2 testing, was distributed to 68 laboratories in 35 countries. Specificity samples included seasonal human coronaviruses hCoV-229E, hCoV-NL63 and hCoV-OC43, as well as MERS-CoV, SARS-CoV and human influenza virus A and B.Conclusion: Sensitivity was variable among laboratories, particularly for low concentration SARS-CoV-2 samples. Results indicated that performance was mostly independent of the selection of specific extraction or PCR methods.
. 2020 Dec 9;JCM.02676-20.
doi: 10.1128/JCM.02676-20. Online ahead of print.
Variable sensitivity in molecular detection of SARS-CoV-2 in European Expert Laboratories: External Quality Assessment, June - July 2020
Carlo Fischer[SUP] 1 [/SUP], Ramona M?gling[SUP] 2 [/SUP], Angeliki Melidou[SUP] 3 [/SUP], Arne K?hne[SUP] 1 [/SUP], Edmilson F Oliveira-Filho[SUP] 1 [/SUP], Thorsten Wolff[SUP] 4 [/SUP], Janine Reiche[SUP] 4 [/SUP], Eeva Broberg[SUP] 3 [/SUP], Adam Meijer[SUP] 2 [/SUP], Katrin Leitmeyer[SUP] 3 [/SUP], Jan Felix Drexler[SUP] 5 [/SUP], Chantal B E M Reusken[SUP] 6 [/SUP]
Affiliations
- PMID: 33298612
- DOI: 10.1128/JCM.02676-20
Abstract
Objective: During the ongoing coronavirus disease 2019 (COVID-19) outbreak, robust detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a key element for clinical management and to interrupt transmission chains. We organized an external quality assessment (EQA) of molecular detection of SARS-CoV-2 for European expert laboratories.Study design: An EQA panel composed of 12 samples, containing either SARS-CoV-2 at different concentrations to evaluate sensitivity or other respiratory viruses to evaluate specificity of SARS-CoV-2 testing, was distributed to 68 laboratories in 35 countries. Specificity samples included seasonal human coronaviruses hCoV-229E, hCoV-NL63 and hCoV-OC43, as well as MERS-CoV, SARS-CoV and human influenza virus A and B.Conclusion: Sensitivity was variable among laboratories, particularly for low concentration SARS-CoV-2 samples. Results indicated that performance was mostly independent of the selection of specific extraction or PCR methods.