Giuseppe
Emeritus
J Clin Microbiol. 2009 Mar 11. [Epub ahead of print]
Universal Detection and Identification of Avian Influenza Using Resequencing Microarrays.
Lin B, Malanoski AP, Wang Z, Blaney KM, Long NC, Meador CE, Metzgar D, Myers CA, Yingst SL, Monteville MR, Saad MD, Schnur JM, Tibbetts C, Stenger DA. - Center for Bio/Molecular Science & Engineering, Code 6900, Naval Research Laboratory, Washington, DC 20375; Nova Research Incorporated, Alexandria, VA 22308; Department of Respiratory Disease Research, Naval Health Research Center, San Diego, CA 92106; Naval Medical Research Unit #3, Cairo, Egypt; Tessarae LLC, Potomac Falls, VA 20165.
Zoonotic microbes have historically been, and continue to emerge as, threats to human health. The recent outbreaks of highly pathogenic avian influenza in bird populations and the appearance of some human infections have increased the concern of a possible new influenza pandemic which highlights the need for broad spectrum detection methods for rapidly identifying the spread or outbreak of all variants of avian influenza virus. In this study, we demonstrate that high-density resequencing pathogen microarrays can be such a tool.
The results from 37 influenza isolates show that the RPM platform is an effective means to detect and subtype influenza, while simultaneously providing sequence information for strain resolution, pathogenicity and drug resistance without additional analysis.
This study establishes that the RPM platform is a broad-spectrum pathogen detection and surveillance tool for monitoring the circulation of prevalent influenza viruses in the poultry industry, wild birds, or incidental exposures and infections in humans.
PMID: 19279171 [PubMed - as supplied by publisher]
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Universal Detection and Identification of Avian Influenza Using Resequencing Microarrays.
Lin B, Malanoski AP, Wang Z, Blaney KM, Long NC, Meador CE, Metzgar D, Myers CA, Yingst SL, Monteville MR, Saad MD, Schnur JM, Tibbetts C, Stenger DA. - Center for Bio/Molecular Science & Engineering, Code 6900, Naval Research Laboratory, Washington, DC 20375; Nova Research Incorporated, Alexandria, VA 22308; Department of Respiratory Disease Research, Naval Health Research Center, San Diego, CA 92106; Naval Medical Research Unit #3, Cairo, Egypt; Tessarae LLC, Potomac Falls, VA 20165.
Zoonotic microbes have historically been, and continue to emerge as, threats to human health. The recent outbreaks of highly pathogenic avian influenza in bird populations and the appearance of some human infections have increased the concern of a possible new influenza pandemic which highlights the need for broad spectrum detection methods for rapidly identifying the spread or outbreak of all variants of avian influenza virus. In this study, we demonstrate that high-density resequencing pathogen microarrays can be such a tool.
The results from 37 influenza isolates show that the RPM platform is an effective means to detect and subtype influenza, while simultaneously providing sequence information for strain resolution, pathogenicity and drug resistance without additional analysis.
This study establishes that the RPM platform is a broad-spectrum pathogen detection and surveillance tool for monitoring the circulation of prevalent influenza viruses in the poultry industry, wild birds, or incidental exposures and infections in humans.
PMID: 19279171 [PubMed - as supplied by publisher]
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