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J Clin Microbiol. Rapid Detection of Influenza A Pandemic (H1N1) 2009 Virus Neuraminidase Resistance Mutation H275Y by Real-Time RT-PCR.

Giuseppe

Giuseppe

Emeritus
Rapid Detection of Influenza A Pandemic (H1N1) 2009 Virus Neuraminidase Resistance Mutation H275Y by Real-Time RT-PCR (J Clin Microbiol., abstract, edited)

[Source: US National Library of Medicine, LINK. EDITED.]

J Clin Microbiol. 2010 Mar 10. [Epub ahead of print]

Rapid Detection of Influenza A Pandemic (H1N1) 2009 Virus Neuraminidase Resistance Mutation H275Y by Real-Time RT-PCR.

Hindiyeh M, Ram D, Mandelboim M, Meningher T, Hirsh S, Robinov J, Levy V,Orzitzer S, Azar R, Grossman Z, Mendelson E. - Central Virology Laboratory, Public Health Services, Ministry of Health, Chaim Sheba Medical Center, Tel-Hashomer Israel; Faculty of Life Sciences, Bar-Ilan University, Ramat Gan Israel; Department of Epidemiology and Preventive Medicine, School of Public Health, Sackler Faculty of Medicine, Tel-Aviv University,Tel-Aviv, Israel.


The emergence of oseltamivir-resistant influenza A pandemic (H1N1) 2009 virus highlights the need for rapid oseltamivir resistance screening. We report the development and validation of high-throughput real-time RT-PCR assays for the detection of the H275Y substitution in neuraminidase 1 gene that can be accomplished in 3-4 hours.

PMID: 20220153 [PubMed - as supplied by publisher]​
 
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