Giuseppe
Emeritus
J Clin Microbiol. 2009 Jul 8. [Epub ahead of print]
Rapid and highly sensitive neuraminidase subtyping of avian influenza viruses by a diagnostic DNA microarray.
Gall A, Hoffmann B, Harder T, Grund C, Ehricht R, Beer M. Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, S?dufer 10, 17493 Greifswald - Insel Riems, Germany; CLONDIAG GmbH, L?bstedter Strasse 103 - 105, 07749 Jena, Germany.
Rapid neuraminidase subtyping of avian influenza viruses from diagnostic samples is crucial considering permanently emerging and evolving strains. Here, we report an easy-to-use, low-cost microarray for neuraminidase subtyping following fragment amplification by a generic, neuraminidase-specific RT-PCR. It enables highly specific characterization with a sensitivity equal to matrix-gene specific real-time RT-PCR.
PMID: 19587298 [PubMed - as supplied by publishe
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Rapid and highly sensitive neuraminidase subtyping of avian influenza viruses by a diagnostic DNA microarray.
Gall A, Hoffmann B, Harder T, Grund C, Ehricht R, Beer M. Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, S?dufer 10, 17493 Greifswald - Insel Riems, Germany; CLONDIAG GmbH, L?bstedter Strasse 103 - 105, 07749 Jena, Germany.
Rapid neuraminidase subtyping of avian influenza viruses from diagnostic samples is crucial considering permanently emerging and evolving strains. Here, we report an easy-to-use, low-cost microarray for neuraminidase subtyping following fragment amplification by a generic, neuraminidase-specific RT-PCR. It enables highly specific characterization with a sensitivity equal to matrix-gene specific real-time RT-PCR.
PMID: 19587298 [PubMed - as supplied by publishe
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